Evidence map›Paper›PMID 37503182›Full record

ArticlebioRxiv : the preprint server for biology2023

A modular chemigenetic calcium indicator enables in vivo functional imaging with near-infrared light.

Helen Farrants, Yichun Shuai, William C Lemon, Christian Monroy Hernandez, Shang Yang, Ronak Patel, Guanda Qiao, Michelle S Frei, Jonathan B Grimm, Timothy L Hanson and 9 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

19 authors.

Helen FarrantsJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Yichun ShuaiJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
William C LemonJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Christian Monroy HernandezDepartment of Neuroscience, Washington University in St. Louis, St. Louis, MO 63110, USA.
Shang YangJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Ronak PatelJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Guanda QiaoDepartment of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.
Michelle S FreiDepartment of Chemical Biology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany.
Jonathan B GrimmJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Timothy L HansonJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Filip TomaskaJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Glenn C TurnerJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Carsen StringerJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Philipp J KellerJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Abraham G BeyeneJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Yao ChenDepartment of Neuroscience, Washington University in St. Louis, St. Louis, MO 63110, USA.
Yajie LiangDepartment of Diagnostic Radiology and Nuclear Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.
Luke D LavisJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Eric R SchreiterJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.

Funding

Spatial, temporal, and context-dependent features of protein kinase A activityR01NS119821 · NINDS · WASHINGTON UNIVERSITY · PI Yao Chen · 2021 to 2026
$2.4M
NINDS NIH HHS R01 NS119821
6 · The paper itself

Abstract

Genetically encoded fluorescent calcium indicators have revolutionized neuroscience and other biological fields by allowing cellular-resolution recording of physiology during behavior. However, we currently lack bright, genetically targetable indicators in the near infrared that can be used in animals. Here, we describe WHaloCaMP, a modular chemigenetic calcium indicator built from bright dye-ligands and protein sensor domains that can be genetically targeted to specific cell populations. Fluorescence change in WHaloCaMP results from reversible quenching of the bound dye via a strategically placed tryptophan. WHaloCaMP is compatible with rhodamine dye-ligands that fluoresce from green to near-infrared, including several dye-ligands that efficiently label the central nervous system in animals. When bound to a near-infrared dye-ligand, WHaloCaMP1a is more than twice as bright as jGCaMP8s, and shows a 7× increase in fluorescence intensity and a 2.1 ns increase in fluorescence lifetime upon calcium binding. We use WHaloCaMP1a with near-infrared fluorescence emission to image Ca

Identifiers

PMID37503182
PMCPMC10370049

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.