Evidence map›Paper›PMID 37503132›Full record

ArticlebioRxiv : the preprint server for biology2023

Methods for making and observing model lipid droplets.

Sonali A Gandhi, Shahnaz Parveen, Munirah Alduhailan, Ramesh Tripathi, Nasser Junedi, Mohammad Saqallah, Matthew A Sanders, Peter M Hoffmann, Katherine Truex, James G Granneman and 1 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Sonali A GandhiDepartment of Physics and Astronomy, Wayne State University, Detroit, MI, USA 48201.
Shahnaz ParveenDepartment of Physics and Astronomy, Wayne State University, Detroit, MI, USA 48201.
Munirah AlduhailanDepartment of Physics and Astronomy, Wayne State University, Detroit, MI, USA 48201.
Ramesh TripathiDepartment of Physics and Astronomy, Wayne State University, Detroit, MI, USA 48201.
Nasser JunediDepartment of Physics and Astronomy, Wayne State University, Detroit, MI, USA 48201.
Mohammad SaqallahDepartment of Physics and Astronomy, Wayne State University, Detroit, MI, USA 48201.
Matthew A SandersCenter for Molecular Medicine and Genetics, School of Medicine, Wayne State University, Detroit, MI, USA 40201.
Peter M HoffmannDepartment of Physics and Astronomy, Wayne State University, Detroit, MI, USA 48201.
Katherine TruexDepartment of Physics, United States Naval Academy, Annapolis, MD, USA 21402.
James G GrannemanCenter for Molecular Medicine and Genetics, School of Medicine, Wayne State University, Detroit, MI, USA 40201.
Christopher V KellyDepartment of Physics and Astronomy, Wayne State University, Detroit, MI, USA 48201.
Wayne State University · USUnited States Naval Academy · US

Funding

Tumor Biology and Microenvironment (Program 1)P30CA022453 · NCI · WAYNE STATE UNIVERSITY · PI Julie Lynn Boerner · 1985 to 2026
$68.4M
Analysis of Lipolytic Trafficking in Adipocytes.R01DK076629 · NIDDK · WAYNE STATE UNIVERSITY · PI James G Granneman, Christopher V Kelly · 2009 to 2026
$7.1M
A stable path for a core based scientist to advance imaging and cytometry in basic and preclinical research at the Karmanos Cancer InstituteR50CA251068 · NCI · WAYNE STATE UNIVERSITY · PI MOIN, KAMIAR · 2020 to 2024
$1.4M
NCI NIH HHS P30 CA022453NCI NIH HHS R50 CA251068NIDDK NIH HHS R01 DK076629
6 · The paper itself

Abstract

The mechanisms by which the lipid droplet (LD) membrane is remodeled in concert with the activation of lipolysis incorporate a complex interplay of proteins, phospholipids, and neutral lipids. Model LDs (mLDs) provide an isolated, purified system for testing the mechanisms by which the droplet composition, size, shape, and tension affects triglyceride metabolism. Described here are methods of making and testing mLDs ranging from 0.1 to 40 μm diameter with known composition. Methods are described for imaging mLDs with high-resolution microscopy during buffer exchanges for the measurement of membrane binding, diffusion, and tension via fluorescence correlation spectroscopy (FCS), fluorescence recovery after photobleaching (FRAP), fluorescence lifetime imaging microscopy (FLIM), atomic force microscopy (AFM), pendant droplet tensiometry, and imaging flow cytometry. These complementary, cross-validating methods of measuring LD membrane behavior reveal the interplay of biophysical processes in triglyceride metabolism.

Indexed as

atomic force microscopyfluorescence correlation spectroscopyfluorescence lifetime imaging microscopyfluorescence microscopyfluorescence recovery after photobleachingModel lipid dropletsphospholipid monolayers

Identifiers

PMID37503132
PMCPMC10370146
OpenAlexW4384826330

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.