Evidence map›Paper›PMID 37502991›Full record

ArticlebioRxiv : the preprint server for biology2023

Handling difficult cryo-ET samples: A study with primary neurons from

Joseph Y Kim, Jie E Yang, Josephine W Mitchell, Lauren A English, Sihui Z Yang, Tanner Tenpas, Erik W Dent, Jill Wildonger, Elizabeth R Wright

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Joseph Y KimDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Jie E YangDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Josephine W MitchellDepartment of Chemistry and Biochemistry, Kalamazoo College, Kalamazoo, MI, 49006, USA.
Lauren A EnglishNeuroscience Training Program, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Sihui Z YangDepartment of Biology, Stanford University, Stanford, CA, 94305, USA.
Tanner TenpasDepartment of Neuroscience, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Erik W DentDepartment of Neuroscience, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Jill WildongerDepartments of Pediatrics and Biological Sciences, University of California, San Diego, La Jolla, CA, 92093, USA.
Elizabeth R WrightDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
University of Wisconsin–Madison · USUniversity of Wisconsin System · USKalamazoo College · USStanford University · USUniversity of California San Diego · US

Funding

The Midwest Center for Cryo-Electron TomographyU24GM139168 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI WRIGHT, ELIZABETH R · 2020 to 2025
$41.7M
Structural Biology of Alpha-Synuclein in Lewy Body DementiaRF1NS110436 · NINDS · WASHINGTON UNIVERSITY · PI KOTZBAUER, PAUL T, RIENSTRA, CHAD M · 2023 to 2023
$4.2M
F-BAR proteins in neuronal migration and process formationR01NS115400 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI DENT, ERIK W · 2021 to 2025
$1.9M
MOLECULAR MOTORS AND NEURONAL MICROTUBULE POLARITYR01NS102385 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI WILDONGER, JILL C · 2017 to 2022
$1.6M
Structural Investigations Of Macromolecular Complexes Critical To hRSV Life CycleR01GM114561 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI SANTANGELO, PHILIP J, WRIGHT, ELIZABETH R · 2016 to 2019
$1.6M
NIGMS NIH HHS R01 GM114561NIGMS NIH HHS U24 GM139168NINDS NIH HHS R01 NS102385NINDS NIH HHS R01 NS115400NINDS NIH HHS RF1 NS110436
6 · The paper itself

Abstract

Cellular neurobiology has benefited from recent advances in the field of cryo-electron tomography (cryo-ET). Numerous structural and ultrastructural insights have been obtained from plunge-frozen primary neurons cultured on electron microscopy grids. With most primary neurons been derived from rodent sources, we sought to expand the breadth of sample availability by using primary neurons derived from 3

Identifiers

PMID37502991
PMCPMC10369871
OpenAlexW4384039635

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.