Long ssRNA undergoes continuous compaction in the presence of polyvalent cations.

Ana Luisa Duran-Meza, Liya Oster, Richard Sportsman, Martin Phillips, Charles M Knobler, William M Gelbart

Abstract read

In one paragraph

Article in Biophysical journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ana Luisa Duran-MezaDepartment of Chemistry and Biochemistry, UCLA, Los Angeles, California.

Liya OsterDepartment of Chemistry and Biochemistry, UCLA, Los Angeles, California.

Richard SportsmanDepartment of Chemistry and Biochemistry, UCLA, Los Angeles, California.

Martin PhillipsDepartment of Chemistry and Biochemistry, UCLA, Los Angeles, California.

Charles M KnoblerDepartment of Chemistry and Biochemistry, UCLA, Los Angeles, California. Electronic address: knobler@chem.ucla.edu.

William M GelbartDepartment of Chemistry and Biochemistry, UCLA, Los Angeles, California; Molecular Biology Institute, UCLA, Los Angeles, California; California NanoSystems Institute, UCLA, Los Angeles, California.

Funding

CHEMISTRY BIOLOGY INTERFACE TRAINING PROGRAMT32GM008496 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MAYNARD, HEATHER D · 1993 to 2019

$6.3M

NIGMS NIH HHS T32 GM008496

6 · The paper itself

Abstract

In the presence of polyvalent cations, long double-stranded DNA (dsDNA) in dilute solution undergoes a single-molecule, first-order, phase transition ("condensation"), a phenomenon that has been documented and analyzed by many years of experimental and theoretical studies. There has been no systematic effort, however, to determine whether long single-stranded RNA (ssRNA) shows an analogous behavior. In this study, using dynamic light scattering, analytical ultracentrifugation, and gel electrophoresis, we examine the effects of increasing polyvalent cation concentrations on the effective size of long ssRNAs ranging from 3000 to 12,000 nucleotides. Our results indicate that ssRNA does not undergo a discontinuous condensation as does dsDNA but rather a "continuous" decrease in size with increasing polyvalent cation concentration. And, instead of the 10-fold decrease in size shown by long dsDNA, we document a 50% decrease, as demonstrated for a range of lengths and sequences of ssRNA.

Indexed as

DNARNACationsCationsDNARNA

Identifiers

PMID37501368

DOI10.1016/j.bpj.2023.07.022

PMCPMC10502455

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.