Evidence map›Paper›PMID 37497554›Full record

ArticlePharmaceutical biology2023

Cardioprotective effects of arjunolic acid in LPS-stimulated H9C2 and C2C12 myotubes via the My88-dependent TLR4 signaling pathway.

Md Mahmudul Hasan, Priya Madhavan, Nur Adelina Ahmad Noruddin, Wai Kwan Lau, Qamar Uddin Ahmed, Aditya Arya, Zainul Amiruddin Zakaria

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pharmaceutical biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Md Mahmudul HasanSchool of Biosciences, Faculty of Health and Medical Sciences, Taylor's University, Subang Jaya, Malaysia.
Priya MadhavanSchool of Medicine, Faculty of Health and Medical Sciences, Taylor's University, Subang Jaya, Malaysia.
Nur Adelina Ahmad NoruddinNational Institutes of Biotechnology Malaysia, Malaysian Institute of Pharmaceuticals and Nutraceuticals, Serdang, Malaysia.
Wai Kwan LauNational Institutes of Biotechnology Malaysia, Malaysian Institute of Pharmaceuticals and Nutraceuticals, Serdang, Malaysia.
Qamar Uddin AhmedDrug Discovery and Synthetic Chemistry Research Group, Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan, Malaysia.
Aditya AryaSchool of Medicine, Faculty of Health and Medical Sciences, Taylor's University, Subang Jaya, Malaysia.
Zainul Amiruddin ZakariaBorneo Research for Algesia, Inflammation and Neurodegeneration (BRAIN) Group, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu, Malaysia.
Taylor's University · MYNational Institutes of Biotechnology Malaysia · MYInternational Islamic University Malaysia · MYUniversiti of Malaysia Sabah · MY

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

contextArjunolic acid (AA) is a triterpenoid saponin found in

objectiveThis study investigates the cardioprotective effects of arjunolic acid (AA) MATERIALS AND

methodsThe MTT viability assay was used to assess the cytotoxicity of AA. LPS induced

resultsAfter 24 h of co-treatment, the expression of TLR4, MyD88, MAPK, JNK, and NF-κB markers were upregulated significantly (2-6 times) in the LPS-treated groups compared to the untreated control in both HCS and WB experiments. Evidently, the HCS analysis revealed that MyD88, NF-κB, p38, and JNK were significantly downregulated in the H9C2 myotube in the AA treated groups. In HCS, the expression of NF-κB was downregulated in C2C12. Additionally, TLR4 expression was downregulated in both H9C2 and C2C12 myotubes in the WB experiment. DISCUSSION AND

conclusionsTLR4 marker expression in H9C2 and C2C12 myotubes was subsequently decreased by AA treatment, suggesting possible cardioprotective effects of AA.

Indexed as

NF-kappa BTriterpenesAnimalsLipopolysaccharidesMiceMuscle Fibers, SkeletalMyeloid Differentiation Factor 88RatsSignal TransductionToll-Like Receptor 4arjunolic acidLipopolysaccharidesMyeloid Differentiation Factor 88NF-kappa BToll-Like Receptor 4TriterpenesC2C12 myotubecardiovascular diseaseH9C2 myotubehigh content screeningMyD88skeletal muscle cell

Identifiers

PMID37497554
PMCPMC10375937
OpenAlexW4385295024

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.