Evidence map›Paper›PMID 37497482›Full record

ArticleBiomedical optics express2023

Two-photon microendoscope for label-free imaging in stereotactic neurosurgery.

Tarah A Welton, Nicholas M George, Baris N Ozbay, Arianna Gentile Polese, Gregory Osborne, Gregory L Futia, J Keenan Kushner, Bette Kleinschmidt-DeMasters, Allyson L Alexander, Aviva Abosch and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 6 institutions in 1 country.

Tarah A WeltonDepartment of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Nicholas M GeorgeNeuroscience Graduate Program, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Baris N OzbayIntelligent Imaging Innovations, Denver, Colorado, 80216, USA.
Arianna Gentile PoleseDepartment of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Gregory OsborneDepartment of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Gregory L FutiaDepartment of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.ORCID https://orcid.org/0000-0003-2266-9525
J Keenan KushnerNeuroscience Graduate Program, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Bette Kleinschmidt-DeMastersDepartment of Pathology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Allyson L AlexanderDepartment of Neurosurgery, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Aviva AboschDepartment of Neurosurgery, University of Nebraska Medical Center, Omaha, NE 68198, USA.
Steven OjemannDepartment of Neurosurgery, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Diego RestrepoDepartment of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Emily A GibsonDepartment of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
University of Colorado Anschutz Medical Campus · USChildren's Hospital Colorado · USIntelligent Light (United States) · USUniversity of Colorado Denver · USUniversity of Colorado System · USUniversity of Nebraska Medical Center · US

Funding

Colorado Clinical and Translational Sciences InstituteUL1TR001082 · NCATS · UNIVERSITY OF COLORADO DENVER · PI SOKOL, RONALD J. · 2013 to 2017
$48.0M
A compact, modular two-photon fiber-coupled microscope for in vivo all-optical electrophysiologyR43MH119879 · NIMH · 3 I · PI GIBSON, EMILY, KILBORN, KARL · 2019 to 2019
$394k
NCATS NIH HHS UL1 TR001082NIMH NIH HHS R43 MH119879
6 · The paper itself

Abstract

We demonstrate a gradient refractive index (GRIN) microendoscope with an outer diameter of ∼1.2 mm and a length of ∼186 mm that can fit into a stereotactic surgical cannula. Two photon imaging at an excitation wavelength of 900 nm showed a field of view of ∼180 microns and a lateral and axial resolution of 0.86 microns and 9.6 microns respectively. The microendoscope was tested by imaging autofluorescence and second harmonic generation (SHG) in label-free human brain tissue. Furthermore, preliminary image analysis indicates that image classification models can predict if an image is from the subthalamic nucleus or the surrounding tissue using conventional, bench-top two-photon autofluorescence.

Identifiers

PMID37497482
PMCPMC10368057
OpenAlexW4380987263

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.