Evidence map›Paper›PMID 37497460›Full record

ArticleBio-protocol2023

Intravital Imaging of Intestinal Intraepithelial Lymphocytes.

Sara McArdle, Goo-Young Seo, Mitchell Kronenberg, Zbigniew Mikulski

Open access · diamondAbstract read
In one paragraph

Article in Bio-protocol, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 68% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Sara McArdleLa Jolla Institute for Immunology; La Jolla, CA, USA.
Goo-Young SeoLa Jolla Institute for Immunology; La Jolla, CA, USA.
Mitchell KronenbergLa Jolla Institute for Immunology; La Jolla, CA, USA.
Zbigniew MikulskiLa Jolla Institute for Immunology; La Jolla, CA, USA.
La Jolla Institute for Immunology · US

Funding

HVEM: A TNF family receptor that influences mucosal immunity and the microbiomeU01AI125955 · NIAID · LA JOLLA INSTITUTE FOR IMMUNOLOGY · PI KRONENBERG, MITCHELL · 2016 to 2020
$2.7M
NIAID NIH HHS U01 AI125955
6 · The paper itself

Abstract

Intestinal intraepithelial lymphocytes (IEL) are a numerous population of T cells located within the epithelium of the small and large intestines, being more numerous in the small intestine (SI). They surveil this tissue by interacting with epithelial cells. Intravital microscopy is an important tool for visualizing the patrolling activity of IEL in the SI of live mice. Most IEL express CD8α; therefore, here we describe an established protocol of intravital imaging that tracks lymphocytes labeled with a CD8α-specific monoclonal antibody in the SI epithelium of live mice. We also describe data acquisition and quantification of the movement metrics, including mean speed, track length, displacement length, and paths for each CD8α

Indexed as

Cell labelingConfocal reflectionIntestineIntraepithelial lymphocyteIntravital microscopySuction ringTracking

Identifiers

PMID37497460
PMCPMC10366999
OpenAlexW4385060273

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.