Evidence map›Paper›PMID 37494933›Full record

ArticleDevelopmental cell2023

Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid droplet regulation.

Melissa A Roberts, Kirandeep K Deol, Alyssa J Mathiowetz, Mike Lange, Dara E Leto, Julian Stevenson, Sayed Hadi Hashemi, David W Morgens, Emilee Easter, Kartoosh Heydari and 6 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed
10.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed, 57 citations in OpenAlex.

  1. Article
  2. CypA is a molecular barrier to HIV emergence from Eastern chimpanzees.bioRxiv : the preprint server for biology · 2026
    Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 6 institutions in 1 country.

Melissa A RobertsDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Nutritional Sciences and Toxicology, University of California, Berkeley, Berkeley, CA 94720, USA.
Kirandeep K DeolDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Nutritional Sciences and Toxicology, University of California, Berkeley, Berkeley, CA 94720, USA.
Alyssa J MathiowetzDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Nutritional Sciences and Toxicology, University of California, Berkeley, Berkeley, CA 94720, USA.
Mike LangeDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Nutritional Sciences and Toxicology, University of California, Berkeley, Berkeley, CA 94720, USA.
Dara E LetoDepartment of Biology, Stanford University, Stanford, CA 94305, USA.
Julian StevensonDepartment of Nutritional Sciences and Toxicology, University of California, Berkeley, Berkeley, CA 94720, USA.
Sayed Hadi HashemiDepartment of Computer Science, University of Illinois at Urbana-Champaign, Urbana, IL 61820, USA.
David W MorgensDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Emilee EasterDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Nutritional Sciences and Toxicology, University of California, Berkeley, Berkeley, CA 94720, USA.
Kartoosh HeydariCancer Research Laboratory FACS Core Facility, University of California, Berkeley, Berkeley, CA 94720, USA.
Mike A NallsData Tecnica International, LLC, Washington, DC, USA; Center for Alzheimer's and Related Dementias, National Institutes of Health, Bethesda, MD 20892, USA; Laboratory of Neurogenetics, National Institute on Aging, National Institutes of Health, Bethesda, MD 20892, USA.
Michael C BassikDepartment of Genetics, Stanford University, Stanford, CA 94305, USA.
Martin KampmannInstitute for Neurodegenerative Diseases, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
Ron R KopitoDepartment of Biology, Stanford University, Stanford, CA 94305, USA.
Faraz FaghriData Tecnica International, LLC, Washington, DC, USA; Center for Alzheimer's and Related Dementias, National Institutes of Health, Bethesda, MD 20892, USA; Laboratory of Neurogenetics, National Institute on Aging, National Institutes of Health, Bethesda, MD 20892, USA.
James A OlzmannDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Nutritional Sciences and Toxicology, University of California, Berkeley, Berkeley, CA 94720, USA; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA. Electronic address: olzmann@berkeley.edu.
University of California, Berkeley · USStanford University · USNational Institutes of Health · USChan Zuckerberg Initiative (United States) · USUniversity of California, San Francisco · USUniversity of Illinois Urbana-Champaign · US

Funding

UCSF Nutrition Obesity Research CenterP30DK098722 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CHRISTIAN VAISSE · 2015 to 2026
$14.6M
The Ubiquitin Proteasome System in Quality ControlR01GM074874 · NIGMS · STANFORD UNIVERSITY · PI KOPITO, RON R · 2006 to 2022
$8.0M
Molecular Mechanisms of Lipid Droplet BiogenesisR01GM112948 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI OLZMANN, JAMES A · 2015 to 2024
$3.1M
The role of UFMylation in ribosome quality control at the ERR01GM148477 · NIGMS · STANFORD UNIVERSITY · PI RON R KOPITO · 2023 to 2026
$2.4M
Lipid droplet regulation and proteome dynamicsR01DK128099 · NIDDK · UNIVERSITY OF CALIFORNIA BERKELEY · PI OLZMANN, JAMES A · 2021 to 2025
$1.6M
Elucidating the Mechanisms of Lipid Droplet Protein DegradationF31DK121477 · NIDDK · UNIVERSITY OF CALIFORNIA BERKELEY · PI ROBERTS, MELISSA · 2019 to 2021
$123k
Genetic interaction (GI) analysis of the mammalian ERAD systemF32GM113370 · NIGMS · STANFORD UNIVERSITY · PI LETO, DARA · 2015 to 2015
$58k
NIDDK NIH HHS F31 DK121477NIDDK NIH HHS P30 DK098722NIDDK NIH HHS R01 DK128099NIGMS NIH HHS F32 GM113370NIGMS NIH HHS R01 GM074874NIGMS NIH HHS R01 GM112948NIGMS NIH HHS R01 GM148477
6 · The paper itself

Abstract

Despite the key roles of perilipin-2 (PLIN2) in governing lipid droplet (LD) metabolism, the mechanisms that regulate PLIN2 levels remain incompletely understood. Here, we leverage a set of genome-edited human PLIN2 reporter cell lines in a series of CRISPR-Cas9 loss-of-function screens, identifying genetic modifiers that influence PLIN2 expression and post-translational stability under different metabolic conditions and in different cell types. These regulators include canonical genes that control lipid metabolism as well as genes involved in ubiquitination, transcription, and mitochondrial function. We further demonstrate a role for the E3 ligase MARCH6 in regulating triacylglycerol biosynthesis, thereby influencing LD abundance and PLIN2 stability. Finally, our CRISPR screens and several published screens provide the foundation for CRISPRlipid (http://crisprlipid.org), an online data commons for lipid-related functional genomics data. Our study identifies mechanisms of PLIN2 and LD regulation and provides an extensive resource for the exploration of LD biology and lipid metabolism.

Indexed as

CRISPR-Cas SystemsLipid DropletsCell LineHumansLipid MetabolismPerilipin-2Perilipin-2PLIN2 protein, humanCRISPRendoplasmic reticulumERADgenetic screenlipid dropletMARCH6metabolismperilipinPLIN2resource

Identifiers

PMID37494933
PMCPMC10530302
OpenAlexW4385248506

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.