ArticlePloS one2023
Genomic alterations involved in fluoroquinolone resistance development in Staphylococcus aureus.
Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 23 citations in OpenAlex.
- Genomic diversity and resistance determinants of staphylococci from cow and buffalo milk.Molecular biology reports · 2026Article
- Trends and drivers of multidrug-resistant bacteria incidence in 59 Chilean intensive care units, 2015-2024: a Bayesian hierarchical analysis.Lancet regional health. Americas · 2026Article
- The Difluoroboranyl-Fluoroquinolone Derivative "7a" Inhibits Bacterial DNA Gyrase and Exhibits Potent Activity Against Ciprofloxacin-ResistantMolecules (Basel, Switzerland) · 2026Article
- Genotypic and Phenotypic Characterization of MRSA Colonization and Persistence in Residential Aged Care Facilities in Adelaide, Australia.International journal of microbiology · 2026Article
- Evaluation of Antimicrobial and Antibiofilm Activity ofMicroorganisms · 2025Article
- Influence of Sub-Inhibitory Concentrations of Sanitizers and Oxacillin on the Resistance of Methicillin-ResistantVeterinary sciences · 2025Article
- Topoisomerase inhibitory activity of ethanolic extracts from botanicalsJournal of medical microbiology · 2025Article
- Staphylococcus saprophyticus prevents skin damage by inhibiting Staphylococcus aureus quorum sensing.Scientific reports · 2025Article
- The Derivative Difluoroboranyl-Fluoroquinolone "7a" Generates Effective Inhibition Against theCurrent issues in molecular biology · 2025Article
- Fluoroquinolone resistance in ESKAPE pathogens: evolutionary pathways, one health transmission, and clinical surveillance.Frontiers in microbiology · 2025Review
- Article
- Identification of novel antistaphylococcal hit compounds.The Journal of antibiotics · 2024Article
- Bacterial Contamination of Environmental Surfaces of Veterinary Rehabilitation Clinics.Animals : an open access journal from MDPI · 2024Article
- Genetic Complexity of CC5Pathogens (Basel, Switzerland) · 2024Article
- Forecasting the development of antimicrobial resistance ofFrontiers in oral health · 2024Article
- Whole genome sequencing distinguishes skin colonizing from infection-associatedFrontiers in cellular and infection microbiology · 2024Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimFluoroquinolone (FQ) is a potent antibiotic class. However, resistance to this class emerges quickly which hinders its application. In this study, mechanisms leading to the emergence of multidrug-resistant (MDR) Staphylococcus aureus (S. aureus) strains under FQ exposure were investigated. METHODOLOGY: S. aureus ATCC 29213 was serially exposed to ciprofloxacin (CIP), ofloxacin (OFL), or levofloxacin (LEV) at sub-minimum inhibitory concentrations (sub-MICs) for 12 days to obtain S. aureus -1 strains and antibiotic-free cultured for another 10 days to obtain S. aureus-2 strains. The whole genome (WGS) and target sequencing were applied to analyze genomic alterations; and RT-qPCR was used to access the expressions of efflux-related genes, alternative sigma factors, and genes involved in FQ resistance.
resultsA strong and irreversible increase of MICs was observed in all applied FQs (32 to 128 times) in all S. aureus-1 and remained 16 to 32 times in all S. aureus-2. WGS indicated 10 noticeable mutations occurring in all FQ-exposed S. aureus including 2 insdel mutations in SACOL0573 and rimI; a synonymous mutation in hslO; and 7 missense mutations located in an untranslated region. GrlA, was found mutated (R570H) in all S. aureus-1 and -2. Genes encoding for efflux pumps and their regulator (norA, norB, norC, and mgrA); alternative sigma factors (sigB and sigS); acetyltransferase (rimI); methicillin resistance (fmtB); and hypothetical protein BJI72_0645 were overexpressed in FQ-exposed strains.
conclusionThe emergence of MDR S. aureus was associated with the mutations in the FQ-target sequences and the overexpression of efflux pump systems and their regulators.
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