ArticleFrontiers in microbiology2023
Rapid identification of bacteria using a multiplex polymerase chain reaction system for acute abdominal infections.
Article in Frontiers in microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 13 citations in OpenAlex.
- Electric-Field-Assisted Enrichment and Electrochemical Detection of Gram-Positive Bacteria Using an FEM-Guided Interdigitated Electrode Biosensor.Biosensors · 2026Article
- Clinical evaluation of multiplex pathogen real-time PCR for early detection of pathogens and antimicrobial resistance genes in intra-abdominal infections.World journal of microbiology & biotechnology · 2026Article
- Evolving Approaches to Bacterial Identification: A Review of Classical and Modern Techniques.International journal of molecular sciences · 2026Review
- Multiplex PCR-Based Detection of Eight Carbapenemase Genes and Their Clinical Characteristics in Urinary Tract Infections.Antibiotics (Basel, Switzerland) · 2026Article
- TRACE: reconstructing fragmented microbial landscapes for high-resolution antimicrobial resistance surveillance.Frontiers in microbiology · 2026Article
- Rapid identification of microbial pathogens in intra-abdominal infections using multiplex PCR.Frontiers in cellular and infection microbiology · 2026Observational
- Controversies in nosocomial peritonitis.Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia · 2025Review
- Identification and characterization of an invasive, hyper-aerotolerantASM case reports · 2025Article
- Multiplex PCR-based rapid pathogen identification in acute cholecystitis using the FilmArray BCID2 panel.Frontiers in microbiology · 2025Article
- Economic Impact in the Treatment of Coagulase-Negative Staphylococci Blood Cultures Contamination in a Middle-Income Country.Revista da Sociedade Brasileira de Medicina Tropical · 2025Article
- The role of rapid multiplex molecular syndromic panels in the clinical management of infections in critically ill patients: an experts-opinion document.Critical care (London, England) · 2024Review
- eHealth and mHealth in Antimicrobial Stewardship to Reduce Mortality in Empirical Antimicrobial Therapy and a Systematic Review with a Meta-Analysis of Adequate Therapy.Infectious disease reports · 2024Review
- eHealth and mHealth in Antimicrobial Stewardship Programs.Digital biomarkersReview
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purpose: Acute abdominal infections can be fatal if the causative organism (s) are misidentified. The spread of antimicrobial-resistant bacteria has become a serious problem worldwide, making antibiotic selection extremely difficult. Using quantitative metagenomic analysis, we evaluated a commercial multiplex polymerase chain reaction (PCR) system (FilmArray™, bioMérieux, Marcy-l'Étoile, France) for the rapid identification of causative bacteria. Methods: The cases of 10 patients with acute abdominal infections were enrolled in this retrospective study. There were six cases of perforated peritonitis and four cases of intraabdominal abscess. Fluid collected from the acute surgical abdominal infections were examined. Results: All specimens tested positive for microorganisms in culture, and six involved two or more microorganisms. Using the multiplex PCR system, nine of ten specimens were found to involve at least one microorganism. One specimen was not included in the multiplex PCR system panel. Nineteen of 21 microorganisms (90.5%) isolated by culture were detected by the multiplex PCR system. Microorganisms with very small numbers of reads (19 reads) were detectable. Conclusion: This multiplex PCR system showed a high detection rate for causative microorganisms in ascites and intraabdominal abscesses. This system may be suitable as an affordable rapid identification system for causative bacteria in these cases.
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