ArticlePlant communications2023
Combining single-cell RNA sequencing with spatial transcriptome analysis reveals dynamic molecular maps of cambium differentiation in the primary and secondary growth of trees.
Article in Plant communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 49 citations in OpenAlex.
- Unlocking the full potential of spatial omics in plants: practical challenges, solutions, and a path forward.The Plant cell · 2026Review
- Article
- PlantCCC prioritizes context-specific candidate ligand-receptor communication patterns in plant spatial transcriptomics.Plant molecular biology · 2026Article
- Functional insights into a putative 2-OG oxygenase involved in jasmonic acid metabolism and xylem development in Populus trichocarpa.Plant & cell physiology · 2026Article
- Cell-Specific Expression and Cellular Compartmental Regulation in Camptothecin Biosynthesis.Plant biotechnology journal · 2026Article
- Single-nucleus RNA-seq and ATAC-seq analyses provide molecular insights into cadmium-stress response in alfalfa roots.Horticulture research · 2026Article
- Serial Spatial Transcriptomes Reveal Regulatory Transitions in Maize Leaf Development.Plant biotechnology journal · 2026Article
- Mass Spectrometry Imaging Combined With Single-Cell Transcriptional Profiling Reveals the Multidimensional Spatial Distributions and Biosynthetic Pathways of Medicinal Components in Andrographis paniculata.Plant biotechnology journal · 2026Article
- Endogenous Multilayer Control of Cambial Stem Cells and Its Consequences for Wood Formation.Plants (Basel, Switzerland) · 2026Review
- Integrating scRNA-seq and snRNA-seq with spatial transcriptomics to unlock the xylem puzzle.Genome biology · 2026Article
- Recent advances inFrontiers in plant science · 2026Review
- Single-Cell Omics in Legumes: Research Trends and Applications.Plants (Basel, Switzerland) · 2025Review
- PagMIR166c targets PagECH2 to regulate cambial differentiation into secondary xylem, cell expansion, and lignin deposition via different auxin signaling pathways in poplar stems.The New phytologist · 2025Article
- Single-nucleus transcriptomics revealed auxin-driven mechanisms of wood plasticity to enhance severe drought tolerance in poplar.Genome biology · 2025Article
- Deep tissue profiling of Populus stem at single nucleus level reveals uncharacterized cell types and cell-specific gene regulatory networks.Genome biology · 2025Article
- Genome-Wide Identification and Evolutionary Analysis of m6A-Related Gene Family in Poplar Nanlin895.Plants (Basel, Switzerland) · 2025Article
- The developmental transcriptome dynamics of current-year shoot utilized as scion in Camellia chekiangoleosa.BMC plant biology · 2025Article
- Advances and applications in single-cell and spatial genomics.Science China. Life sciences · 2025Review
- A single-cell and spatial wheat root atlas with cross-species annotations delineates conserved tissue-specific marker genes and regulators.Cell reports · 2025Article
- Investigating biological nitrogen fixation via single-cell transcriptomics.Journal of experimental botany · 2025Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Primary and secondary growth of the tree stem are responsible for corresponding increases in trunk height and diameter. However, our molecular understanding of the biological processes that underlie these two types of growth is incomplete. In this study, we used single-cell RNA sequencing and spatial transcriptome sequencing to reveal the transcriptional landscapes of primary and secondary growth tissues in the Populus stem. Comparison between the cell atlas and differentiation trajectory of primary and secondary growth revealed different regulatory networks involved in cell differentiation from cambium to xylem precursors and phloem precursors. These regulatory networks may be controlled by auxin accumulation and distribution. Analysis of cell differentiation trajectories suggested that vessel and fiber development followed a sequential pattern of progressive transcriptional regulation. This research provides new insights into the processes of cell identity and differentiation that occur throughout primary and secondary growth of tree stems, increasing our understanding of the cellular differentiation dynamics that occur during stem growth in trees.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.