ArticleThe New phytologist2023
An improved Nicotiana benthamiana bioproduction chassis provides novel insights into nicotine biosynthesis.
Article in The New phytologist, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Polyphenol oxidase depletion in Nicotiana benthamiana enhances recombinant protein purification and preserves native protein integrity.The New phytologist · 2026Article
- Nicotine biosynthesis is completed by cryptic activating glucosylation.Nature communications · 2026Article
- Duckweeds: from fundamental biology to a sustainable plant chassis for biotechnology.Advanced biotechnology · 2026Review
- Integrated transcriptomic and metabolomic analyses reveal the regulatory network underlying NtGSTU10-mediated nicotine synthesis and transport in tobacco.Scientific reports · 2026Article
- Heterologous Production of Forskolin in Tobacco (Nicotiana tabacum) via Glandular Trichome Specific Engineering and Metabolic Flux Redirection.Plant biotechnology journal · 2026Article
- Harnessing Bulk-Segregant Mapping to Identify Trait-Associated Genes in the Allopolyploid Model Plant Nicotiana benthamiana.Plant biotechnology journal · 2026Article
- Complete nicotine biosynthesis reshapes the understanding of a classic alkaloid metabolic pathway.Frontiers in plant science · 2026Article
- Localising enzymes to biomolecular condensates increases their accumulation and benefits engineered metabolic pathway performance in Nicotiana benthamiana.Plant biotechnology journal · 2026Article
- Optimized expression of human interleukin-15 inBiotechnology reports (Amsterdam, Netherlands) · 2025Article
- EfficientInternational journal of molecular sciences · 2025Article
- Navigating the challenges of engineering composite specialized metabolite pathways in plants.The Plant journal : for cell and molecular biology · 2025Review
- Establishing a comprehensive web-based analysis platform for Nicotiana benthamiana genome and transcriptome.The Plant journal : for cell and molecular biology · 2025Article
- Development of an RNA virus vector for non-transgenic genome editing in tobacco and generation ofaBIOTECH · 2024Article
- Expression of kiwifruit-derived actinidin inFrontiers in plant science · 2024Article
- Evolutionary metabolomics of specialized metabolism diversification in the genusScience advances · 2023Article
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
Abstract
The model plant Nicotiana benthamiana is an increasingly attractive organism for the production of high-value, biologically active molecules. However, N. benthamiana accumulates high levels of pyridine alkaloids, in particular nicotine, which complicates the downstream purification processes. Here, we report a new assembly of the N. benthamiana genome as well as the generation of low-nicotine lines by CRISPR/Cas9-based inactivation of berberine bridge enzyme-like proteins (BBLs). Triple as well as quintuple mutants accumulated three to four times less nicotine than the respective control lines. The availability of lines without functional BBLs allowed us to probe their catalytic role in nicotine biosynthesis, which has remained obscure. Notably, chiral analysis revealed that the enantiomeric purity of nicotine was fully lost in the quintuple mutants. In addition, precursor feeding experiments showed that these mutants cannot facilitate the specific loss of C6 hydrogen that characterizes natural nicotine biosynthesis. Our work delivers an improved N. benthamiana chassis for bioproduction and uncovers the crucial role of BBLs in the stereoselectivity of nicotine biosynthesis.
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