Evidence map›Paper›PMID 37488620›Full record

ArticleCancer cell international2023

The effect of matrices on the gene expression profile of patient-derived head and neck carcinoma cells for in vitro therapy testing.

Aini Hyytiäinen, Katja Korelin, Mervi Toriseva, Tommy Wilkman, Satu Kainulainen, Karri Mesimäki, Johannes Routila, Sami Ventelä, Heikki Irjala, Matthias Nees and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell international, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 7 institutions in 1 country.

Aini HyytiäinenDepartment of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland.
Katja KorelinDepartment of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland.
Mervi TorisevaInstitute of Biomedicine, University of Turku, Turku, 20520, Finland.
Tommy WilkmanDepartment of Oral and Maxillofacial Diseases, Helsinki University Hospital, Helsinki, Finland.
Satu KainulainenDepartment of Oral and Maxillofacial Diseases, Helsinki University Hospital, Helsinki, Finland.
Karri MesimäkiDepartment of Oral and Maxillofacial Diseases, Helsinki University Hospital, Helsinki, Finland.
Johannes RoutilaFICAN West Cancer Centre, University of Turku and Turku University Hospital, Turku, Finland.
Sami VenteläFICAN West Cancer Centre, University of Turku and Turku University Hospital, Turku, Finland.
Heikki IrjalaDepartment of Otorhinolaryngology - Head and Neck surgery, Turku University Hospital and University of Turku, Turku, Finland.
Matthias NeesInstitute of Biomedicine, University of Turku, Turku, 20520, Finland.
Ahmed Al-Samadi *Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland.
Tuula Salo *Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland. tuula.salo@helsinki.fi.
University of Helsinki · FITurku University Hospital · FIUniversity of Turku · FIÅbo Akademi University · FIHelsinki University Hospital · FIHospital District of Helsinki and Uusimaa · FIUniversity of Eastern Finland · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveHead and neck squamous cell carcinoma (HNSCC) is a highly aggressive tumor with a 5-year mortality rate of ~ 50%. New in vitro methods are needed for testing patients' cancer cell response to anti-cancer treatments. We aimed to investigate how the gene expression of fresh carcinoma tissue samples and freshly digested single cancer cells change after short-term cell culturing on plastic, Matrigel or Myogel. Additionally, we studied the effect of these changes on the cancer cells' response to anti-cancer treatments. MATERIALS/

methodsFresh tissue samples from HNSCC patients were obtained perioperatively and single cells were enzymatically isolated and cultured on either plastic, Matrigel or Myogel. We treated the cultured cells with cisplatin, cetuximab, and irradiation; and performed cell viability measurement. RNA was isolated from fresh tissue samples, freshly isolated single cells and cultured cells, and RNA sequencing transcriptome profiling and gene set enrichment analysis were performed.

resultsCancer cells obtained from fresh tissue samples changed their gene expression regardless of the culturing conditions, which may be due to the enzymatic digestion of the tissue. Myogel was more effective than Matrigel at supporting the upregulation of pathways related to cancer cell proliferation and invasion. The impacts of anti-cancer treatments varied between culturing conditions.

conclusionsOur study showed the challenge of in vitro cancer drug testing using enzymatic cell digestion. The upregulation of many targeted pathways in the cultured cells may partially explain the common clinical failure of the targeted cancer drugs that pass the in vitro testing.

Indexed as

Gene expressionHead and neck cancerIn vitro 3DPatient-derived cellsTumor microenvironment

Identifiers

PMID37488620
PMCPMC10367262
OpenAlexW4385210304

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.