Evidence map›Paper›PMID 37488440›Full record

ArticlePlanta2023

Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole.

Vincenzo D'Amelia, Julien Curaba, Muhammad Ali Abid, Salvatore Esposito, Pablo Cavagnaro, Domenico Carputo, Massimo Iorizzo

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 3 countries.

Vincenzo D'Amelia *Institute of Bioscience and BioResources, National Research Council, Via Università 100, 80055, Portici, Italy.ORCID http://orcid.org/0000-0002-8548-6825
Julien Curaba *Horticulture Science Department, Plants for Human Health Institute, North Carolina State University, North Carolina Research Campus, Kannapolis, NC, USA.
Muhammad Ali AbidHorticulture Science Department, Plants for Human Health Institute, North Carolina State University, North Carolina Research Campus, Kannapolis, NC, USA.
Salvatore EspositoCREA Research Centre for Cereal and Industrial Crops (CREA-CI), 71122, Foggia, Italy.ORCID http://orcid.org/0000-0002-2785-1468
Pablo CavagnaroNational Scientific and Technical Research Council (CONICET), National Institute of Agricultural Technology (INTA) E.E.A. La Consulta, La Consulta CC8, San Carlos, 5567, Mendoza, Argentina.ORCID http://orcid.org/0000-0001-5838-0876
Domenico CarputoDepartment of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055, Portici, Italy.
Massimo IorizzoHorticulture Science Department, Plants for Human Health Institute, North Carolina State University, North Carolina Research Campus, Kannapolis, NC, USA. miorizz@ncsu.edu.ORCID http://orcid.org/0000-0002-0032-8247
North Carolina State University · USCereal Research Centre · ITConsejo Nacional de Investigaciones Científicas y Técnicas · ARInstitute of Biosciences and Bioresources · ITUniversity of Naples Federico II · IT

Funding

National Institute of Food and Agriculture 2022-67013-36389Università degli Studi di Napoli Federico II 2017Università degli Studi di Napoli Federico II STAR-Line 1
6 · The paper itself

Abstract

MAIN

conclusionDcMYB11, an R2R3 MYB gene associated with petiole anthocyanin pigmentation in carrot, was functionally characterized. A putative enhancer sequence is able to increase DcMYB11 activity. The accumulation of anthocyanin pigments can exhibit different patterns across plant tissues and crop varieties. This variability allowed the investigation of the molecular mechanisms behind the biosynthesis of these pigments in several plant species. Among crops, carrots have a well-defined anthocyanin pigmentation pattern depending on the genic background. In this work, we report on the discovery of DNA structural differences affecting the activity of an R2R3 MYB (encoded by DcMYB11) involved in anthocyanin regulation in carrot petiole. To this end, we first verified the function of DcMYB11 using heterologous systems and identified three different alleles which may explain differences in petiole pigmentation. Characterization of the DcMYB11 alleles at the 5' upstream sequence unveiled a sequence that functions as a putative enhancer. In conclusion, this study provides novel insight into the molecular mechanisms controlling anthocyanin accumulation in carrot. By these outcomes, we expanded our knowledge on the cis-regulatory sequences in plants.

Indexed as

Daucus carotaAllelesAnthocyaninsCrops, AgriculturalPigmentationAnthocyaninsAnthocyaninsCarrot petioleDaucus carotaRegulatory sequencesTranscription factor

Identifiers

PMID37488440
OpenAlexW4385197545

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.