Evidence map›Paper›PMID 37488390›Full record

ArticleAnnals of surgical oncology2023

Detecting Somatic Mutations for Well-Differentiated Pancreatic Neuroendocrine Tumors in Endoscopic Ultrasound-Guided Fine Needle Aspiration with Next-Generation Sequencing.

Elie M Ghabi, Joseph R Habib, Sami Shoucair, Ammar A Javed, Jonathan Sham, William R Burns, John L Cameron, Syed Z Ali, Eun Ji Shin, Paolo Giorgio Arcidiacono and 5 more

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Article in Annals of surgical oncology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 2 countries.

Elie M GhabiDepartment of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Joseph R HabibDepartment of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Sami ShoucairDepartment of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Ammar A JavedDepartment of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Jonathan ShamDepartment of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
William R BurnsDepartment of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
John L CameronDepartment of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Syed Z AliDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Eun Ji ShinDepartment of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Paolo Giorgio ArcidiaconoPancreato-Biliary Endoscopy and Endosonography Division, Pancreas Translational and Clinical Research Center, San Raffaele Scientific Institute IRCCS, Vita Salute San Raffaele University, Milan, Italy.
Claudio DoglioniPathology Unit, Pancreas Translational and Clinical Research Center, IRCCS Ospedale San Raffaele, ENETS Center of Excellence, Milan, Italy.
Massimo FalconiPancreatic and Transplant Surgery Unit, Pancreas Translational and Clinical Research Center, IRCCS Ospedale San Raffaele, ENETS Center of Excellence, Milan, Italy.
Jun Yu *Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Stefano Partelli *Pancreatic and Transplant Surgery Unit, Pancreas Translational and Clinical Research Center, IRCCS Ospedale San Raffaele, ENETS Center of Excellence, Milan, Italy.
Jin He *Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA. jhe11@jhmi.edu.ORCID http://orcid.org/0000-0001-9149-2247
Johns Hopkins University · USIRCCS Ospedale San Raffaele · ITVita-Salute San Raffaele University · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPancreatic neuroendocrine tumors (PanNETs) exhibit heterogenous behavior, whereby some small tumors are aggressive with a propensity for metastasis. Detection of somatic mutations associated with aggressive biology may help with patient stratification and surgical decision-making in patients with well-differentiated PanNETs. Using next-generation sequencing (NGS), we investigated the feasibility of detecting somatic mutations in endoscopic ultrasound-guided, fine-needle aspiration (EUS-FNA) specimens and determining the mutational concordance between the EUS-FNA specimens and the primary tumors.

methodsThirty-eight patients with well-differentiated, nonfunctioning PanNETs were obtained from two tertiary referral centers. Patient demographic characteristics and tumor, clinicopathologic features were collected. Tissue from both the EUS-FNA specimen and the primary tumor was extracted from archival tissue blocks. NGS using a panel of ten genes was performed on both samples.

resultsIn our series, the median age was 61.1 years. Tumors were predominantly left-sided (60.5%) and unifocal (94.7%). The median tumor size was 2.2 cm. NGS detected somatic mutations in 29% of primary tumors and 36.8% of EUS-FNA specimens. In primary tumors, DAXX/ATRX mutations were predominantly detected (63.6%). In EUS-FNA specimens, MEN1 mutations were predominantly detected (64.3%). Among non-wild-type specimens, mutational concordance was achieved in 31.6% of cases. In 11 patients with a detectable mutation in the primary tumor, a mutation was detected in the EUS-FNA specimen in 45.5% of cases, with a mutational concordance of 54.5%.

conclusionsNGS can detect somatic mutations in EUS-FNA specimens of well-differentiated PanNETs. Efforts to improve detection sensitivity and mutational concordance are required to overcome current technical limitations.

Indexed as

Biomarkers, TumorEndoscopic Ultrasound-Guided Fine Needle AspirationHigh-Throughput Nucleotide SequencingMutationNeuroendocrine TumorsPancreatic NeoplasmsAdultAgedCell DifferentiationFemaleFollow-Up StudiesHumansMaleMiddle AgedPrognosisBiomarkers, Tumor

Identifiers

PMID37488390
OpenAlexW4385197560

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.