Evidence map›Paper›PMID 37488082›Full record

ArticleZhongguo fei ai za zhi = Chinese journal of lung cancer2023

CTCs Detection and Whole-exome Sequencing Might Be Used to Differentiate Benign and Malignant Pulmonary Nodules.

Changdan Xu, Xiaohong Xu, Weipeng Shao, Hongliang Sun, Xiaohong Liu, Hongxiang Feng, Xianbo Zuo, Jingyang Gao, Guohui Wang, Xiongtao Yang and 5 more

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Article in Zhongguo fei ai za zhi = Chinese journal of lung cancer, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Changdan XuDepartment of Radiation Oncology, Peking University China-Japan Friendship School of Clinical Medicine, Beijing 100029, China.
Xiaohong XuDepartment of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Weipeng ShaoDepartment of Thoracic Surgery, China-Japan Friendship Hospital, Beijing 100029, China.
Hongliang SunDepartment of Radiology, China-Japan Friendship Hospital, Beijing 100029, China.
Xiaohong LiuDepartment of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Hongxiang FengDepartment of Thoracic Surgery, China-Japan Friendship Hospital, Beijing 100029, China.
Xianbo ZuoDepartment of Dermatology, China-Japan Friendship Hospital, Beijing 100029, China.
Jingyang GaoDepartment of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Guohui WangDepartment of Radiation Oncology, Tianjin First Central Hospital, Tianjin 300070, China.
Xiongtao YangDepartment of Oncology, Beijing Changping District Hospital, Beijing 102200, China.
Runchuan GuFaculty of Medicine Lund University, Lund SE-22100, Sweden.
Shutong GeDepartment of Radiation Oncology, Peking University China-Japan Friendship School of Clinical Medicine, Beijing 100029, China.
Shijie WangDepartment of Radiation Oncology, China-Japan Friendship Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100029, China.
Liwei GaoDepartment of Radiation Oncology, Center of Respiratory Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
Guangying ZhuDepartment of Radiation Oncology, Peking University China-Japan Friendship School of Clinical Medicine, Beijing 100029, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLow-density computed tomography (LDCT) improved early lung cancer diagnosis but introduces an excess of false-positive pulmonary nodules data. Hence, accurate diagnosis of early-stage lung cancer remains challenging. The purpose of the study was to assess the feasibility of using circulating tumour cells (CTCs) to differentiate malignant from benign pulmonary nodules.

methods122 patients with suspected malignant pulmonary nodules detected on chest CT in preparation for surgery were prospectively recruited. Peripheral blood samples were collected before surgery, and CTCs were identified upon isolation by size of epithelial tumour cells and morphological analysis. Laser capture microdissection, MALBAC amplification, and whole-exome sequencing were performed on 8 samples. The diagnostic efficacy of CTCs counting, and the genomic variation profile of benign and malignant CTCs samples were analysed.

resultsUsing 2.5 cells/5 mL as the cut-off value, the area under the receiver operating characteristic curve was of 0.651 (95% confidence interval: 0.538-0.764), with a sensitivity and specificity of 0.526 and 0.800, respectively, and positive and negative predictive values of 91.1% and 30.3%, respectively. Distinct sequence variations differences in DNA damage repair-related and driver genes were observed in benign and malignant samples. TP53 mutations were identified in CTCs of four malignant cases; in particular, g.7578115T>C, g.7578645C>T, and g.7579472G>C were exclusively detected in all four malignant samples.

conclusionsCTCs play an ancillary role in the diagnosis of pulmonary nodules. TP53 mutations in CTCs might be used to identify benign and malignant pulmonary nodules.

Indexed as

CarcinomaLung NeoplasmsMultiple Pulmonary NodulesDNA RepairExome SequencingHumansChest computed tomographyCirculating tumour cellsLung noduleTP53Whole-exome sequencing

Identifiers

PMID37488082
PMCPMC10365965

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