ArticleThe Plant cell2023
Allelic compatibility in plant immune receptors facilitates engineering of new effector recognition specificities.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 38 citations in OpenAlex.
- Baiting blast fungi: an engineered NLR domain improves effector recognition.The Plant cell · 2026Article
- Structure-informed engineering of plant-microbe interactions.The Plant journal : for cell and molecular biology · 2026Review
- Natural variation inScience advances · 2026Article
- Engineering an Exo70 integrated domain of a barley NLR for improved blast resistance.The Plant cell · 2026Article
- How to frustrate a plant pathogen.The Plant journal : for cell and molecular biology · 2026Review
- Dual recognition of structurally unrelated mildew effectors underlies the broad-spectrum resistance of Pm3e in wheat.Nature communications · 2026Article
- Plant NLRs are getting into higher-order architectures.The Plant journal : for cell and molecular biology · 2026Review
- Unraveling plant immunity: from pathogen perception to resistance engineering.Science China. Life sciences · 2026Review
- Mismatch screening in Nicotiana benthamiana to explore Pik-1/Pik-2 paired NLR platforms for receptor engineering.The New phytologist · 2026Article
- Precision Editing of NLRS Improves Effector Recognition for Enhanced Disease Resistance.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- TheProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Structural Insights Into the Role of RxLR Effectors in the Arms Race Between Oomycetes and Plants.Molecular plant pathology · 2025Review
- Genomics-driven discovery of superior alleles and genes for yellow rust resistance in wheat.Nature genetics · 2025Article
- A root-specific NLR network mediates immune signaling of resistance genes against plant parasitic nematodes.The Plant cell · 2025Article
- The resistance awakens: Diversity at the DNA, RNA, and protein levels informs engineering of plant immune receptors from Arabidopsis to crops.The Plant cell · 2025Review
- Helper NLR immune protein NRC3 evolved to evade inhibition by a cyst nematode virulence effector.PLoS genetics · 2025Article
- Resurrection of the Plant Immune Receptor Sr50 to Overcome Pathogen Immune Evasion.bioRxiv : the preprint server for biology · 2025Article
- Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors.The Plant cell · 2024Article
- Bioengineering a plant NLR immune receptor with a robust binding interface toward a conserved fungal pathogen effector.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
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13 authors at 2 institutions in 2 countries.
Funding
Abstract
Engineering the plant immune system offers genetic solutions to mitigate crop diseases caused by diverse agriculturally significant pathogens and pests. Modification of intracellular plant immune receptors of the nucleotide-binding leucine-rich repeat (NLR) receptor superfamily for expanded recognition of pathogen virulence proteins (effectors) is a promising approach for engineering disease resistance. However, engineering can cause NLR autoactivation, resulting in constitutive defense responses that are deleterious to the plant. This may be due to plant NLRs associating in highly complex signaling networks that coevolve together, and changes through breeding or genetic modification can generate incompatible combinations, resulting in autoimmune phenotypes. The sensor and helper NLRs of the rice (Oryza sativa) NLR pair Pik have coevolved, and mismatching between noncoevolved alleles triggers constitutive activation and cell death. This limits the extent to which protein modifications can be used to engineer pathogen recognition and enhance disease resistance mediated by these NLRs. Here, we dissected incompatibility determinants in the Pik pair in Nicotiana benthamiana and found that heavy metal-associated (HMA) domains integrated in Pik-1 not only evolved to bind pathogen effectors but also likely coevolved with other NLR domains to maintain immune homeostasis. This explains why changes in integrated domains can lead to autoactivation. We then used this knowledge to facilitate engineering of new effector recognition specificities, overcoming initial autoimmune penalties. We show that by mismatching alleles of the rice sensor and helper NLRs Pik-1 and Pik-2, we can enable the integration of synthetic domains with novel and enhanced recognition specificities. Taken together, our results reveal a strategy for engineering NLRs, which has the potential to allow an expanded set of integrations and therefore new disease resistance specificities in plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.