ArticlemBio2023
A combined microscopy and single-cell sequencing approach reveals the ecology, morphology, and phylogeny of uncultured lineages of zoosporic fungi.
Article in mBio, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- The Origin and Early Evolution of Fungi: Challenges, Inferences, and Principles.Annual review of cell and developmental biology · 2026Review
- Microsporidia: evolution, infection mechanisms and host impact.Nature reviews. Microbiology · 2026Review
- Single-Thallus Genomics of Ejectosporus trisporus, an Unculturable Stonefly Gut Fungal Symbiont.Environmental microbiology · 2026Article
- Exploring the diversity and community structure of the Testudines fecal mycobiome.bioRxiv : the preprint server for biology · 2026Article
- The diversity and abundance of chytrids on the Greenland Ice Sheet.Scientific reports · 2026Article
- Salinity and diatom host community jointly shape chytrid parasite diversity along a natural salinity gradient.ISME communications · 2026Article
- Single-cell genomics, metagenomics, and transcriptomics ofAquatic ecology · 2026Article
- Phytoplankton Under Pressure: Temperature, Precipitation and Cyanobacterial Blooms as Drivers of Chytrid Infections.Environmental microbiology reports · 2025Article
- Non-Dikarya Fungal Clades Are Everywhere: What 18S rRNA Gene Metabarcoding Reveals About Cross-System Distribution of Fungi.Microbial ecology · 2025Article
- ParAquaSeq, a Database of Ecologically Annotated rRNA Sequences Covering Zoosporic Parasites Infecting Aquatic Primary Producers in Natural and Industrial Systems.Molecular ecology resources · 2025Article
- Dynamics of zoosporic parasites in summer phytoplankton communities of the Baltic Sea.FEMS microbiology ecology · 2025Article
- Warming increases richness and shapes assemblages of eukaryotic parasitic plankton.Environmental microbiome · 2025Article
- Exploring Environmental Microfungal Diversity Through Serial Single Cell Screening.Molecular ecology resources · 2025Article
- Thirty novel fungal lineages: formal description based on environmental samples and DNA.MycoKeys · 2025Article
- The first single-cell sequencing ofFrontiers in microbiology · 2025Article
- Article
- Potential for Use of Species in the SubfamilyMicroorganisms · 2024Review
- A review of the taxonomic diversity, host-parasite interactions, and experimental research on chytrids that parasitize diatoms.Frontiers in microbiology · 2023Review
- Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Environmental DNA analyses of fungal communities typically reveal a much larger diversity than can be ascribed to known species. Much of this hidden diversity lies within undescribed fungal lineages, especially the early diverging fungi (EDF). Although these EDF often represent new lineages even at the phylum level, they have never been cultured, making their morphology and ecology uncertain. One of the methods to characterize these uncultured fungi is a single-cell DNA sequencing approach. In this study, we established a large data set of single-cell sequences of EDF by manually isolating and photographing parasitic fungi on various hosts such as algae, protists, and micro-invertebrates, combined with subsequent long-read sequencing of the ribosomal DNA locus (rDNA). We successfully obtained rDNA sequences of 127 parasitic fungal cells, which clustered into 71 phylogenetic lineages belonging to seven phylum-level clades of EDF: Blastocladiomycota, Chytridiomycota, Aphelidiomycota, Rozellomycota, and three unknown phylum-level clades. Most of our single cells yielded novel sequences distinguished from both described taxa and existing metabarcoding data, indicating an expansive and hidden diversity of parasitic taxa of EDF. We also revealed an unexpected diversity of endobiotic
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