Evidence map›Paper›PMID 37484710›Full record

ArticleNature physics2019

The mechanical stability of proteins regulates their translocation rate into the cell nucleus.

Elvira Infante, Andrew Stannard, Stephanie J Board, Palma Rico-Lastres, Fani Panagaki, Amy E M Beedle, Vinoth Sundar Rajan, Elena Rostkova, Ainhoa Lezamiz, Yong Jian Wang and 4 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature physics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 53 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Review
  11. Article
  12. Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Molten globule-like transition state of protein barnase measured with calorimetric force spectroscopy.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  18. Review
  19. Mechanical Forces in Nuclear Organization.Cold Spring Harbor perspectives in biology · 2022
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 2 countries.

Elvira Infante *Department of Physics and Randall Centre for Cell and Molecular Biophysics, King's College London, WC2R 2LS, London, UK.
Andrew Stannard *Department of Physics and Randall Centre for Cell and Molecular Biophysics, King's College London, WC2R 2LS, London, UK.
Stephanie J BoardDepartment of Physics and Randall Centre for Cell and Molecular Biophysics, King's College London, WC2R 2LS, London, UK.
Palma Rico-LastresDepartment of Physics and Randall Centre for Cell and Molecular Biophysics, King's College London, WC2R 2LS, London, UK.
Fani PanagakiDepartment of Physics and Randall Centre for Cell and Molecular Biophysics, King's College London, WC2R 2LS, London, UK.
Amy E M BeedleDepartment of Physics and Randall Centre for Cell and Molecular Biophysics, King's College London, WC2R 2LS, London, UK.
Vinoth Sundar RajanDepartment of Physics and Randall Centre for Cell and Molecular Biophysics, King's College London, WC2R 2LS, London, UK.
Elena RostkovaDepartment of Physics and Randall Centre for Cell and Molecular Biophysics, King's College London, WC2R 2LS, London, UK.
Ainhoa LezamizDepartment of Physics and Randall Centre for Cell and Molecular Biophysics, King's College London, WC2R 2LS, London, UK.
Yong Jian WangDepartment of Physics and Randall Centre for Cell and Molecular Biophysics, King's College London, WC2R 2LS, London, UK.
Samuel Gulaidi BreenDepartment of Physics and Randall Centre for Cell and Molecular Biophysics, King's College London, WC2R 2LS, London, UK.
Catherine ShanahanCardiovascular Division, James Black Centre, King's College London, London SE5 9NU, UK Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and.
Pere Roca-CusachsTechnology (BIST) and University of Barcelona, 08028 Barcelona, Spain.
Sergi Garcia-ManyesDepartment of Physics and Randall Centre for Cell and Molecular Biophysics, King's College London, WC2R 2LS, London, UK.
King's College London · GBBarcelona Institute of Science and Technology · ES

Funding

Wellcome Trust 210887/Z/18/Z
6 · The paper itself

Abstract

The translocation of mechanosensitive transcription factors (TFs) across the nuclear envelope is a crucial step in cellular mechanotransduction. Yet the molecular mechanisms by which external mechanical cues control the nuclear shuttling dynamics of TFs through the nuclear pore complex (NPC) to activate gene expression are poorly understood. Here, we show that the nuclear import rate of myocardin-related transcription factor A (MRTFA) - a protein that regulates cytoskeletal dynamics via the activation of the TF serum response factor (SRF) - inversely correlates with the protein's nanomechanical stability and does not relate to its thermodynamic stability. Tagging MRTFA with mechanically resistant proteins results in the downregulation of SRF-mediated myosin light-chain 9 (MYL9) gene expression and subsequent slowing down of cell migration. We conclude that the mechanical unfolding of proteins regulates their nuclear translocation rate through the NPC, and highlight the role of the NPC as a selective mechanosensor able to discriminate forces as low as ~10 pN. The modulation of the mechanical stability of TFs may represent a new strategy for the control of gene expression.

Identifiers

PMID37484710
PMCPMC7614795
OpenAlexW2953720032

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.