Evidence map›Paper›PMID 37478288›Full record

ArticleCurrent protocols2023

Murine Models of Salmonella Infection.

Gregory T Walker, Romana R Gerner, Sean-Paul Nuccio, Manuela Raffatellu

Open access · hybridAbstract read
In one paragraph

Article in Current protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
5.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 27 citations in OpenAlex.

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  16. Vitamin D3 contribution to systemic and local control ofFrontiers in molecular biosciences · 2026
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  17. Article
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  19. Article
  20. Single-cell quantification of the microbiota by flow cytometry: MicFLY.bioRxiv : the preprint server for biology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Gregory T WalkerDivision of Host-Microbe Systems & Therapeutics, Department of Pediatrics, University of California San Diego, La Jolla, California.
Romana R GernerDivision of Host-Microbe Systems & Therapeutics, Department of Pediatrics, University of California San Diego, La Jolla, California.
Sean-Paul NuccioDivision of Host-Microbe Systems & Therapeutics, Department of Pediatrics, University of California San Diego, La Jolla, California.
Manuela RaffatelluDivision of Host-Microbe Systems & Therapeutics, Department of Pediatrics, University of California San Diego, La Jolla, California.
University of California, San Diego · USWeihenstephan-Triesdorf University of Applied Sciences · DE

Funding

RESEARCH IN INFECTIOUS DISEASEST32AI007036 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NIZET, VICTOR · 1985 to 2025
$9.2M
C3-Dependent Intracellular Killing in Innate Immunity and Bacterial PathogenesisR01AI145325 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NIZET, VICTOR, RAFFATELLU, MANUELA · 2019 to 2023
$3.1M
Harnessing iron acquisition to hinder enterobacterial pathogenesisR01AI176390 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Elizabeth M Nolan, Manuela Raffatellu · 2023 to 2026
$2.9M
Nutritional immunity during Salmonella infectionR01AI126277 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI RAFFATELLU, MANUELA · 2016 to 2020
$1.9M
Development of siderophore-based vaccines against non-typhoidal Salmonella infectionR01AI114625 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NOLAN, ELIZABETH M, RAFFATELLU, MANUELA · 2015 to 2019
$1.9M
Nutritional immunity during Salmonella infectionR37AI126277 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Manuela Raffatellu · 2023 to 2026
$1.8M
Antimicrobial activity of Escherichia coli Nissle 1917 microcin MR21AI154644 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NOLAN, ELIZABETH M, RAFFATELLU, MANUELA · 2020 to 2021
$440k
Harnessing iron acquisition to hinder enterobacterial pathogenesisR56AI114625 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NOLAN, ELIZABETH M, RAFFATELLU, MANUELA · 2022 to 2022
$400k
NIAID NIH HHS R01 AI114625NIAID NIH HHS R01 AI126277NIAID NIH HHS R01 AI145325NIAID NIH HHS R01 AI176390NIAID NIH HHS R21 AI154644NIAID NIH HHS R37 AI126277NIAID NIH HHS R56 AI114625NIAID NIH HHS T32 AI007036NIH HHS AI114625NIH HHS AI126277NIH HHS AI145325NIH HHS AI154644NIH HHS T32AI007036
6 · The paper itself

Abstract

The pathogen Salmonella enterica encompasses a range of bacterial serovars that cause intestinal inflammation and systemic infections in humans. Mice are a widely used infection model due to their relative simplicity and versatility. Here, we provide standardized protocols for culturing the prolific zoonotic pathogen S. enterica serovar Typhimurium for intragastric inoculation of mice to model colitis or systemic dissemination, along with techniques for direct extraintestinal infection. Furthermore, we present procedures for quantifying pathogen burden and for characterizing the immune response by analyzing tissue pathology, inflammatory markers, and immune cells from intestinal tissues. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Murine colitis model utilizing oral streptomycin pretreatment and oral S. Typhimurium administration Basic Protocol 2: Intraperitoneal injection of S. Typhimurium for modeling extraintestinal infection Support Protocol 1: Preparation of S. Typhimurium inoculum Support Protocol 2: Preparation of mixed S. Typhimurium inoculum for competitive infection Basic Protocol 3: Assessment of S. Typhimurium burden Support Protocol 3: Preservation and pathological assessment of S. Typhimurium-infected tissues Support Protocol 4: Measurement of inflammatory marker expression in intestinal tissues by qPCR Support Protocol 5: Preparation of intestinal content for inflammatory marker quantification by ELISA Support Protocol 6: Immune cell isolation from Salmonella-infected intestinal tissues.

Indexed as

ColitisSalmonella InfectionsAnimalsDisease Models, AnimalHumansIntestinesMiceSalmonella typhimuriumcolitisinfectionmucosaSalmonella

Identifiers

PMID37478288
PMCPMC10372748
OpenAlexW4385064348

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.