Evidence map›Paper›PMID 37477743›Full record

ArticleBulletin of experimental biology and medicine2023

Identification of Chimeric NTRK3 Genes in Papillary Thyroid Cancer Cells by Analyzing the Imbalance of the Expression of 5' and 3' mRNA Fragments.

I P Oskorbin, A A Ivanov, M A Smertina, I A Demidova, U A Boyarskikh, A A Kechin, S Yu Bakharev, O V Samuilenkova, I V Vihlyanov, N E Kushlinskii and 1 more

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Article in Bulletin of experimental biology and medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 59% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Molecular and clinicopathological characteristics ofHistology and histopathology · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

I P OskorbinInstitute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
A A IvanovAltai Regional Oncological Center, Barnaul, Russia.
M A SmertinaInstitute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
I A DemidovaMoscow City Oncology Hospital No. 62, Moscow Healthcare Department, Moscow, Russia.
U A BoyarskikhInstitute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
A A KechinInstitute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
S Yu BakharevAltai Regional Oncological Center, Barnaul, Russia.
O V SamuilenkovaAltai Regional Oncological Center, Barnaul, Russia.
I V VihlyanovAltai Regional Oncological Center, Barnaul, Russia.
N E KushlinskiiN. N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia.
M L FilipenkoInstitute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. mlfilipenko@gmail.com.
Institute of Chemical Biology and Fundamental Medicine · RUSiberian Branch of the Russian Academy of Sciences · RUMinistry of Health of the Russian Federation · RUMoscow City Oncology Hospital №62 · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The standard for detecting chimeric genes of neurotrophic receptor tyrosine kinases (NTRK) is next generation sequencing (NGS). However, this analysis is expensive and takes several days. As a rapid screening method for the detection of NTRK3-dependent papillary thyroid cancer, an analysis of the expression imbalance between 5' and 3' NTRK3 mRNA fragments was used (5'/3' RT-PCR). The reference method for detection of NTRK3 rearrangements was fluorescent in situ hybridization (FISH), and the most frequent rearrangements in papillary thyroid cancer were tested using reverse transcription PCR (RT-PCR). Using 5'/3' RT-PCR, 18 samples of papillary thyroid cancer carrying chimeric transcripts of NTRK3 mRNA were detected. The sensitivity of the developed technique was 88.9% and specificity was 99.3%. Thus, a fast and cost-effective method of screening samples of papillary thyroid cancer in paraffin blocks is proposed with acceptable sensitivity and specificity.

Indexed as

Thyroid NeoplasmsHumansIn Situ Hybridization, FluorescenceReceptor Protein-Tyrosine KinasesRNA, MessengerThyroid Cancer, PapillaryReceptor Protein-Tyrosine KinasesRNA, Messengerchimeric mRNANTRK3papillary thyroid cancerRT-PCRTRK

Identifiers

PMID37477743
OpenAlexW4384925884

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.