Dual-amplification system based on CRISPR-Cas12a and horseradish peroxidase-tethered magnetic microspheres for colorimetric detection of microcystin-LR.

Pian Wu, Man Zhang, Xiaoting Xue, Ping Ding, Lei Ye

Open access · hybridAbstract read

In one paragraph

Article in Mikrochimica acta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

1.5field-weighted citation impact, top 19% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 16 citations in OpenAlex.

Colorimetric aptasensing of microcystin-LR using DNA-conjugated polydiacetylene.Analytical and bioanalytical chemistry · 2024
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Pian WuDivision of Pure and Applied Biochemistry, Department of Chemistry, Lund University, 22100, Lund, Sweden.

Man ZhangDivision of Pure and Applied Biochemistry, Department of Chemistry, Lund University, 22100, Lund, Sweden.

Xiaoting XueDivision of Pure and Applied Biochemistry, Department of Chemistry, Lund University, 22100, Lund, Sweden.

Ping DingXiang Ya School of Public Health, Central South University, Changsha, 410078, Hunan, China. pingshui@csu.edu.cn.

Lei YeDivision of Pure and Applied Biochemistry, Department of Chemistry, Lund University, 22100, Lund, Sweden. Lei.Ye@tbiokem.lth.se.ORCID http://orcid.org/0000-0002-3646-4072

Lund University · SECentral South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A novel dual-amplification system based on CRISPR-Cas12a and horseradish peroxidase (HRP) was developed for colorimetric determination of MC-LR. This dual-amplification was accomplished by combining the nuclease activity of CRISPR-Cas12a with the redox activity of HRP. HRP linked to magnetic beads through an ssDNA (MB-ssDNA-HRP) was used to induce a color change of the 3,3',5,5'-tetramethylbenzidine (TMB)-H

Indexed as

ColorimetryCRISPR-Cas SystemsDNA, ComplementaryHorseradish PeroxidaseHydrogen PeroxideMagnetic PhenomenaMarine ToxinsMicrocystinsMicrospherescyanoginosin LRDNA, ComplementaryHorseradish PeroxidaseHydrogen PeroxideMarine ToxinsMicrocystinsColorimetric detectionCRISPR-Cas12aHorseradish peroxidaseMicrocystin-LR

Identifiers

PMID37474872

DOI10.1248/cpb.30.4114

PMCPMC10359370

OpenAlexW4384924070

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.