ArticleNature methods2023
Voltage-Seq: all-optical postsynaptic connectome-guided single-cell transcriptomics.
Article in Nature methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 13 citations in OpenAlex.
- The basolateral amygdala decodes visceral pain and itch via distinct circuits and molecules.Nature communications · 2026Article
- Optical Recordings of Unitary Synaptic Connections Reveal High and Random Local Connectivity between CA3 Pyramidal Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- High-speed voltage imaging of action potentials in molecular layer interneurons reveals sensory-driven synchrony that augments movement.Cell reports · 2025Article
- A scalable, all-optical method for mapping synaptic connectivity with cell-type specificity.bioRxiv : the preprint server for biology · 2025Article
- All-optical voltage imaging-guided postsynaptic single-cell transcriptome profiling with Voltage-Seq.Nature protocols · 2024Review
- Tonically active GABAergic neurons in the dorsal periaqueductal gray control instinctive escape in mice.Current biology : CB · 2024Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
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Abstract
Understanding the routing of neuronal information requires the functional characterization of connections. Neuronal projections recruit large postsynaptic ensembles with distinct postsynaptic response types (PRTs). PRT is typically probed by low-throughput whole-cell electrophysiology and is not a selection criterion for single-cell RNA-sequencing (scRNA-seq). To overcome these limitations and target neurons based on specific PRTs for soma harvesting and subsequent scRNA-seq, we created Voltage-Seq. We established all-optical voltage imaging and recorded the PRT of 8,347 neurons in the mouse periaqueductal gray (PAG) evoked by the optogenetic activation of ventromedial hypothalamic (VMH) terminals. PRTs were classified and spatially resolved in the entire VMH-PAG connectome. We built an onsite analysis tool named VoltView to navigate soma harvesting towards target PRTs guided by a classifier that used the VMH-PAG connectome database as a reference. We demonstrated Voltage-seq by locating VMH-driven γ-aminobutyric acid-ergic neurons in the PAG, guided solely by the onsite classification in VoltView.
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