ArticleNature microbiology2023
A molecular mechanosensor for real-time visualization of appressorium membrane tension in Magnaporthe oryzae.
Article in Nature microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- A nematode-trapping fungus orchestrates polarity cues, septins, and NOX signaling for trap formation.The Journal of cell biology · 2026Article
- Lipid Bilayer-Confined J-Aggregation Transduces Cell Membrane Mechanics into Photoacoustic Signals.Journal of the American Chemical Society · 2026Article
- MoCox6 is a regulator of mitophagy and a druggable target in Magnaporthe oryzae.Nature microbiology · 2026Article
- β-Xylosidase Overexpression Alters Pectin and Cellulose Distribution and Modulates Blast Disease Resistance in Rice.Plants (Basel, Switzerland) · 2026Article
- The diverse roles of host membranes in plant-microbe interactions.PLoS pathogens · 2026Article
- The role of plant cell walls in pest resistance: current insights and future perspectives.Journal of experimental botany · 2025Review
- Establishment of Novel and Efficient Methods for Investigating Sexual Reproduction inJournal of fungi (Basel, Switzerland) · 2025Article
- The Egh16-like virulence factor TrsA of the nematode-trapping fungus Arthrobotrys flagrans facilitates intrusion into its host Caenorhabditis elegans.PLoS pathogens · 2025Article
- Ultrasensitive imaging-based sensor unlocked by differential guided-mode resonance.Nature communications · 2025Article
- Live cell imaging of plant infection provides new insight into the biology of pathogenesis by the rice blast fungus Magnaporthe oryzae.Journal of microscopy · 2025Review
- Membrane fluidity control by the Magnaporthe oryzae acyl-CoA binding protein sets the thermal range for host rice cell colonization.PLoS pathogens · 2024Article
- The vacuolar fusion regulated by HOPS complex promotes hyphal initiation and penetration in Candida albicans.Nature communications · 2024Article
- Multi-omics analysis revealed that the protein kinase MoKin1 affected the cellular response to endoplasmic reticulum stress in the rice blast fungus, Magnaporthe oryzae.BMC genomics · 2024Article
- Septin-dependent invasive growth by the rice blast fungusJournal of plant diseases and protection : scientific journal of the German Phytomedical Society (DPG) · 2024Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
The rice blast fungus Magnaporthe oryzae uses a pressurized infection cell called an appressorium to drive a rigid penetration peg through the leaf cuticle. The vast internal pressure of an appressorium is very challenging to investigate, leaving our understanding of the cellular mechanics of plant infection incomplete. Here, using fluorescence lifetime imaging of a membrane-targeting molecular mechanoprobe, we quantify changes in membrane tension in M. oryzae. We show that extreme pressure in the appressorium leads to large-scale spatial heterogeneities in membrane mechanics, much greater than those observed in any cell type previously. By contrast, non-pathogenic melanin-deficient mutants, exhibit low spatially homogeneous membrane tension. The sensor kinase ∆sln1 mutant displays significantly higher membrane tension during inflation of the appressorium, providing evidence that Sln1 controls turgor throughout plant infection. This non-invasive, live cell imaging technique therefore provides new insight into the enormous invasive forces deployed by pathogenic fungi to invade their hosts, offering the potential for new disease intervention strategies.
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