ArticleNature communications2023
Fungal small RNAs ride in extracellular vesicles to enter plant cells through clathrin-mediated endocytosis.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 115 citations in OpenAlex.
- Extracellular Vesicle Isolation from Botrytis cinerea Culture and B. cinerea-Infected Arabidopsis Plants.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Advancing the adoption of RNA interference-based biopesticides.Nature plants · 2026Review
- BcMFSR Is Required for Efficient Uptake of Exogenous dsRNA and Spray-Induced Gene Silencing in Botrytis cinerea.Molecular plant pathology · 2026Article
- Long noncoding RNAs cross kingdoms to broaden host-pathogen dialogue.Cell research · 2026Article
- Nano-enabled spray-induced RNA interference against fungal pathogens of oilseed crops: carrier performance, delivery evidence and field translation.World journal of microbiology & biotechnology · 2026Review
- Fungal MicroRNA-Like RNAs Facilitate Convergent Attack on a Host Immune Signalling Network.Plant, cell & environment · 2026Article
- Transcriptomic characterization of Coccidioides morphological states using RiboMarker-enhanced RNA sequencing.G3 (Bethesda, Md.) · 2026Article
- Trans-species microRNAs from the parasitic plant Cuscuta campestris specifically avoid loading onto self Argonautes.Nucleic acids research · 2026Article
- Extracellular Vesicles: Key Mediators of Bioactive Molecule Transport in Plant-Microbe Interactions.Molecular plant pathology · 2026Review
- RNA regulation in plants.Science China. Life sciences · 2026Review
- FungiSNC: An integrated database for fungi small non-coding RNAs based on high-throughput sequencing.Journal of advanced research · 2026Article
- Advancements in extracellular vesicle research.Extracellular vesicle · 2026Article
- Localization and Functional Analysis of CtLTP8, an Extracellular Vesicle Protein That Enhances Resistance toPlants (Basel, Switzerland) · 2026Article
- Stage-Specific RNA Turnover Drives Small RNA Dynamics inPlant direct · 2026Article
- A rice ceRNA module suppresses Rhizoctonia solani-induced cross-kingdom RNAi to reduce fungal pathogenicity.Nature communications · 2026Article
- Cross-kingdom RNA interference promotes arbuscular mycorrhiza development.Nature plants · 2026Article
- Exploring the roles of fungal extracellular vesicles in fungal pathogenesis and symbiosis: Current understandings and future perspectives.World journal of microbiology & biotechnology · 2026Review
- Cross-kingdom RNAi: a universal mechanism of inter-organismal communication with many unknowns.Journal of experimental botany · 2026Review
- Yeast secreted protein PgSCP interacts with citrus transcription factors CsFAR1 to enhance green mold resistance in fruit.Horticulture research · 2026Article
- Natural Variations of ZmRLR1 Mediate the Root Lodging Resistance of Maize by Regulating Root Ascorbate and Auxin Homeostasis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
16 more citing papers are in PubMed but not listed here.
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7 authors at 2 institutions in 2 countries.
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Abstract
Small RNAs (sRNAs) of the fungal pathogen Botrytis cinerea can enter plant cells and hijack host Argonaute protein 1 (AGO1) to silence host immunity genes. However, the mechanism by which these fungal sRNAs are secreted and enter host cells remains unclear. Here, we demonstrate that B. cinerea utilizes extracellular vesicles (EVs) to secrete Bc-sRNAs, which are then internalized by plant cells through clathrin-mediated endocytosis (CME). The B. cinerea tetraspanin protein, Punchless 1 (BcPLS1), serves as an EV biomarker and plays an essential role in fungal pathogenicity. We observe numerous Arabidopsis clathrin-coated vesicles (CCVs) around B. cinerea infection sites and the colocalization of B. cinerea EV marker BcPLS1 and Arabidopsis CLATHRIN LIGHT CHAIN 1, one of the core components of CCV. Meanwhile, BcPLS1 and the B. cinerea-secreted sRNAs are detected in purified CCVs after infection. Arabidopsis knockout mutants and inducible dominant-negative mutants of key components of the CME pathway exhibit increased resistance to B. cinerea infection. Furthermore, Bc-sRNA loading into Arabidopsis AGO1 and host target gene suppression are attenuated in those CME mutants. Together, our results demonstrate that fungi secrete sRNAs via EVs, which then enter host plant cells mainly through CME.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.