Evidence map›Paper›PMID 37470813›Full record

ArticleAnalytical and bioanalytical chemistry2023

Mayaro virus detection by integrating sample preparation with isothermal amplification in portable devices.

Morteza Alipanah, Carlos Manzanas, Xin Hai, John A Lednicky, Alberto Paniz-Mondolfi, J Glenn Morris, Z Hugh Fan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Development of a Platform forAerosol science and technology : the journal of the American Association for Aerosol Research · 2026
    Article
  3. Article
  4. Article
  5. Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Morteza AlipanahInterdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of Florida, P.O. Box 116250, Gainesville, FL, 32611, USA.
Carlos ManzanasInterdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of Florida, P.O. Box 116250, Gainesville, FL, 32611, USA.
Xin HaiInterdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of Florida, P.O. Box 116250, Gainesville, FL, 32611, USA.
John A LednickyEmerging Pathogens Institute, University of Florida, P.O. Box 100009, Gainesville, FL, 32610, USA. jlednicky@phhp.ufl.edu.
Alberto Paniz-MondolfiDepartment of Pathology, Molecular and Cell-Based Medicine, Icahn School of Medicine at Mount Sinai, 1425 Madison Ave, New York, NY, 10029, USA.
J Glenn MorrisEmerging Pathogens Institute, University of Florida, P.O. Box 100009, Gainesville, FL, 32610, USA.
Z Hugh FanInterdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of Florida, P.O. Box 116250, Gainesville, FL, 32611, USA. hfan@ufl.edu.

Funding

Multiplexed Airborne Virus Collection and Detection at the Point-of-CareR01AI158868 · NIAID · UNIVERSITY OF FLORIDA · PI FAN, Z. HUGH · 2021 to 2025
$1.6M
Multiplexed Detection of Mosquito-Borne Viruses at the Point-of-CareR01AI155735 · NIAID · UNIVERSITY OF FLORIDA · PI FAN, Z. HUGH · 2021 to 2024
$1.5M
NIAID NIH HHS R01 AI155735NIAID NIH HHS R01 AI158868
6 · The paper itself

Abstract

Mayaro virus (MAYV) is an emerging mosquito-borne alphavirus that causes clinical symptoms similar to those caused by Chikungunya virus (CHIKV), Dengue virus (DENV), and Zika virus (ZIKV). To differentiate MAYV from these viruses diagnostically, we have developed a portable device that integrates sample preparation with real-time, reverse-transcription, loop-mediated isothermal amplification (rRT-LAMP). First, we designed a rRT-LAMP assay targeting MAYV's non-structural protein (NS1) gene and determined the limit of detection of at least 10 viral genome equivalents per reaction. The assay was specific for MAYV, without cross-reactions with CHIKV, DENV, or ZIKV. The rRT-LAMP assay was integrated with a sample preparation device (SPD) wherein virus lysis and RNA enrichment/purification were carried out on the spot, without requiring pipetting, while subsequent real-time amplification device (RAD) enables virus detection at the point of care (POC). The functions of our platform were demonstrated using purified MAYV RNA or blood samples containing viable viruses. We have used the devices for detection of MAYV in as short as 13 min, with limit of detection to as low as 10 GEs/reaction.

Indexed as

Chikungunya virusZika VirusZika Virus InfectionAnimalsGenome, ViralHumansNucleic Acid Amplification TechniquesRNA, ViralRNA, ViralMayaro virusPoint of careReal-time amplificationrRT-LAMPValve

Identifiers

PMID37470813
PMCPMC10528734

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.