ArticleParasites & vectors2023
Comparative transcriptome profiling of Eimeria tenella in various developmental stages and functional analysis of an ApiAP2 transcription factor exclusively expressed during sporogony.
Article in Parasites & vectors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Genomic structures of rDNA clusters in Eimeria acervulina.BMC genomics · 2026Article
- Molecular mechanisms of surface antigen suppression by ApiAP2 and its implications for vaccine development.Veterinary research · 2025Article
- Genetic manipulation for the non-model protozoaniScience · 2025Review
- Article
- Combined transcriptome and whole genome sequencing analyses reveal candidate drug-resistance genes ofiScience · 2025Article
- Identification of highly cross-reactive immunogens in Eimeria tenella sporozoites.Parasite (Paris, France) · 2025Article
- Application of CRISPR/Cas gene editing for infectious disease control in poultry.Open life sciences · 2025Review
- Transcriptome-wide mapping of internal mRNA NParasites & vectors · 2024Article
- Identification and function characterization of NcAP2XII-4 in Neospora caninum.Parasites & vectors · 2024Article
- RNA-Seq of Phenotypically DistinctPathogens (Basel, Switzerland) · 2023Article
- LocalizationFrontiers in cellular and infection microbiology · 2023Article
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Authors and funding
15 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundThe apicomplexan parasites Eimeria spp. are the causative agents of coccidiosis, a disease with a significant global impact on the poultry industry. The complex life cycle of Eimeria spp. involves exogenous (sporogony) and endogenous (schizogony and gametogony) stages. Unfortunately, the genetic regulation of these highly dynamic processes, particularly for genes involved in specific developmental phases, is not well understood.
methodsIn this study, we used RNA sequencing (RNA-Seq) analysis to identify expressed genes and differentially expressed genes (DEGs) at seven time points representing different developmental stages of Eimeria tenella. We then performed K-means clustering along with co-expression analysis to identify functionally enriched gene clusters. Additionally, we predicted apicomplexan AP2 transcription factors in E. tenella using bioinformatics methods. Finally, we generated overexpression and knockout strains of ETH2_0411800 to observe its impact on E. tenella development.
resultsIn total, we identified 7329 genes that are expressed during various developmental stages, with 3342 genes exhibiting differential expression during development. Using K-means clustering along with co-expression analysis, we identified clusters functionally enriched for oocyte meiosis, cell cycle, and signaling pathway. Among the 53 predicted ApiAP2 transcription factors, ETH2_0411800 was found to be exclusively expressed during sporogony. The ETH2_0411800 overexpression and knockout strains did not exhibit significant differences in oocyst size or output compared to the parental strain, while the resulting ETH2_0411800 knockout parasite showed a relatively small oocyst output.
conclusionsThe findings of our research suggest that ETH2_0411800 is not essential for the growth and development of E. tenella. Our study provides insights into the gene expression dynamics and is a valuable resource for exploring the roles of transcription factor genes in regulating the development of Eimeria parasites.
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