Evidence map›Paper›PMID 37468483›Full record

ArticleNature communications2023

Spatially controlled construction of assembloids using bioprinting.

Julien G Roth, Lucia G Brunel, Michelle S Huang, Yueming Liu, Betty Cai, Sauradeep Sinha, Fan Yang, Sergiu P Pașca, Sungchul Shin, Sarah C Heilshorn

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.

0numbers the graph read from it
0cells of the map it votes in
69citing papers in PubMed
12.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

69 citing papers in PubMed, 117 citations in OpenAlex.

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  11. New developments and applications of human organoids.Nature reviews. Molecular cell biology · 2026
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9 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Julien G RothInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA.ORCID 0000-0002-7560-3258
Lucia G Brunel *Department of Chemical Engineering, Stanford University, Stanford, CA, USA.ORCID 0000-0003-0327-5635
Michelle S Huang *Department of Chemical Engineering, Stanford University, Stanford, CA, USA.ORCID 0000-0002-1814-7786
Yueming LiuDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
Betty CaiDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
Sauradeep SinhaDepartment of Bioengineering, Stanford University, Stanford, CA, USA.
Fan YangDepartment of Bioengineering, Stanford University, Stanford, CA, USA.
Sergiu P PașcaStanford Brain Organogenesis, Wu Tsai Neurosciences Institute & Bio-X, Stanford University, Stanford, CA, USA.ORCID 0000-0002-3216-3248
Sungchul ShinDepartment of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
Sarah C HeilshornStanford Brain Organogenesis, Wu Tsai Neurosciences Institute & Bio-X, Stanford University, Stanford, CA, USA. heilshorn@stanford.edu.ORCID 0000-0002-9801-6304
Stanford University · USNeurosciences Institute · US

Funding

Engineered biomaterials to modulate cell-cell signaling for the robust expansion of stem cellsR01EB027171 · NIBIB · STANFORD UNIVERSITY · PI HEILSHORN, SARAH C · 2019 to 2022
$1.4M
An Engineered Hydrogel Platform to Improve Neural Organoid Reproducibility for a Multi-Organoid Disease Model of 22q11.2 Deletion SyndromeF31NS132505 · NINDS · STANFORD UNIVERSITY · PI HUANG, MICHELLE S · 2023 to 2025
$113k
NIBIB NIH HHS R01 EB027171NINDS NIH HHS F31 NS132505
6 · The paper itself

Abstract

The biofabrication of three-dimensional (3D) tissues that recapitulate organ-specific architecture and function would benefit from temporal and spatial control of cell-cell interactions. Bioprinting, while potentially capable of achieving such control, is poorly suited to organoids with conserved cytoarchitectures that are susceptible to plastic deformation. Here, we develop a platform, termed Spatially Patterned Organoid Transfer (SPOT), consisting of an iron-oxide nanoparticle laden hydrogel and magnetized 3D printer to enable the controlled lifting, transport, and deposition of organoids. We identify cellulose nanofibers as both an ideal biomaterial for encasing organoids with magnetic nanoparticles and a shear-thinning, self-healing support hydrogel for maintaining the spatial positioning of organoids to facilitate the generation of assembloids. We leverage SPOT to create precisely arranged assembloids composed of human pluripotent stem cell-derived neural organoids and patient-derived glioma organoids. In doing so, we demonstrate the potential for the SPOT platform to construct assembloids which recapitulate key developmental processes and disease etiologies.

Indexed as

BioprintingPluripotent Stem CellsBiocompatible MaterialsHumansHydrogelsOrganoidsBiocompatible MaterialsHydrogels

Identifiers

PMID37468483
PMCPMC10356773
OpenAlexW4384819025

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.