Evidence map›Paper›PMID 37467493›Full record

ArticleACS biomaterials science & engineering2023

Aptamer-Based Chromatographic Methods for Efficient and Economical Separation of Leukocyte Populations.

Melissa Ling, Ian I Cardle, Kefan Song, Alexander J Yan, Nataly Kacherovsky, Michael C Jensen, Suzie H Pun

Open access · greenAbstract read
In one paragraph

Article in ACS biomaterials science & engineering, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Melissa LingMolecular Engineering and Sciences Institute, University of Washington, Seattle, Washington 98195, United States.
Ian I CardleDepartment of Bioengineering, University of Washington, Seattle, Washington 98195, United States.ORCID 0000-0001-9000-2293
Kefan SongDepartment of Bioengineering, University of Washington, Seattle, Washington 98195, United States.ORCID 0000-0002-8925-0094
Alexander J YanDepartment of Bioengineering, University of Washington, Seattle, Washington 98195, United States.
Nataly KacherovskyDepartment of Bioengineering, University of Washington, Seattle, Washington 98195, United States.
Michael C JensenSeattle Children's Therapeutics, Seattle, Washington 98101, United States.
Suzie H PunMolecular Engineering and Sciences Institute, University of Washington, Seattle, Washington 98195, United States.ORCID 0000-0003-1443-4996
University of Washington · US

Funding

Interdisciplinary Tranining in Cancer ResearchT32CA080416 · NCI · UNIVERSITY OF WASHINGTON · PI STODDARD, BARRY L. · 1998 to 2023
$9.7M
Traceless, aptamer-based cell isolation for cell therapy applicationsR01EB034235 · NIBIB · UNIVERSITY OF WASHINGTON · PI Suzie H. Pun · 2023 to 2026
$1.4M
NCI NIH HHS T32 CA080416NIBIB NIH HHS R01 EB034235
6 · The paper itself

Abstract

The manufacturing process of chimeric antigen receptor T cell therapies includes isolation systems that provide pure T cells. Current magnetic-activated cell sorting and immunoaffinity chromatography methods produce desired cells with high purity and yield but require expensive equipment and reagents and involve time-consuming incubation steps. Here, we demonstrate that aptamers can be employed in a continuous-flow resin platform for both depletion of monocytes and selection of CD8

Indexed as

CD8-Positive T-LymphocytesLeukocytes, MononuclearChromatographyLeukocytesaptamersCAR T cell manufacturingcell isolationchromatography

Identifiers

PMID37467493
PMCPMC11016351
OpenAlexW4384817735

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.