Evidence map›Paper›PMID 37464247›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Maintenance of Human Primordial Germ Cell-Like Cells in a Long-Term Culture System.

Joanna J Gell, Toshi Shioda

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 78% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Joanna J GellThe Jackson Laboratory for Genomic Medicine, Farmington, CT, USA. Joanna.gell@jax.org.
Toshi ShiodaMassachusetts General Hospital Center for Cancer Research and Harvard Medical School, Charlestown, MA, USA.
Jackson Laboratory · USMassachusetts General Hospital · US

Funding

Applications of the long-term culture human primordial germ cell-like cells to toxicological assessments and mechanistic studies on chemically caused heritable human health threatsR21ES034468 · NIEHS · MASSACHUSETTS GENERAL HOSPITAL · PI SHIODA, TOSHIHIRO · 2023 to 2024
$459k
NIEHS NIH HHS R21 ES034468
6 · The paper itself

Abstract

Primordial germ cells (PGCs) are the earliest form of mammalian germ lineage. In humans, PGCs are present during a very early and limited window in development, limiting the ability to study fundamental developmental steps in human reproductive biology. However, recent advancements in generating in-vitro models of gametogenesis have allowed the field to generate human primordial germ cell-like cells (hPGCLCs). In this chapter, we will review the generation of hPGCLCs using the incipient mesoderm-like cell (iMeLC) protocol and the subsequent expansion of hPGCLCs in a long-term culture system.

Indexed as

Induced Pluripotent Stem CellsAnimalsCell DifferentiationGametogenesisGerm CellsHumansMammalsHuman primordial germ cell-like cellsIn-vitro gametogenesisLong-term culturePluripotent stem cellsPrimordial germ cell

Identifiers

PMID37464247
OpenAlexW4384663893

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.