Evidence map›Paper›PMID 37464244›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

A Roadmap for Three-Dimensional Analysis of the Intact Mouse Ovary.

Bikem Soygur, Mariko H Foecke, Eliza A Gaylord, Adam Fries, Jing Li, Ripla Arora, Diana J Laird

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Review
  9. Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Bikem SoygurDepartment of Obstetrics, Gynecology and Reproductive Science, Center for Reproductive Sciences, Eli and Edythe Broad Center for Regeneration Medicine and Stem Cell Research, UCSF, San Francisco, CA, USA.
Mariko H Foecke *Department of Obstetrics, Gynecology and Reproductive Science, Center for Reproductive Sciences, Eli and Edythe Broad Center for Regeneration Medicine and Stem Cell Research, UCSF, San Francisco, CA, USA.
Eliza A Gaylord *Department of Obstetrics, Gynecology and Reproductive Science, Center for Reproductive Sciences, Eli and Edythe Broad Center for Regeneration Medicine and Stem Cell Research, UCSF, San Francisco, CA, USA.
Adam FriesGenomics and Cell Characterization Core Facility, Institute of Molecular Biology, Eugene, OR, USA.
Jing LiDepartment of Obstetrics, Gynecology and Reproductive Science, Center for Reproductive Sciences, Eli and Edythe Broad Center for Regeneration Medicine and Stem Cell Research, UCSF, San Francisco, CA, USA.
Ripla AroraInstitute for Quantitative Health Science and Engineering, Department of Obstetrics, Gynecology and Reproductive Biology, Michigan State University, East Lansing, MI, USA.
Diana J LairdDepartment of Obstetrics, Gynecology and Reproductive Science, Center for Reproductive Sciences, Eli and Edythe Broad Center for Regeneration Medicine and Stem Cell Research, UCSF, San Francisco, CA, USA. diana.laird@ucsf.edu.

Funding

UCSF Environmental Research and Translation for Health Center (EaRTH Center)P30ES030284 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JENNIFER C FUNG, Tracey J. Woodruff · 2020 to 2026
$11.2M
PREDOCTORAL TRAINING IN DEVELOPMENTAL BIOLOGYT32HD007470 · NICHD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Todd Nystul · 1994 to 2026
$7.7M
Cell competition in the developing mouse germlineDP2OD007420 · OD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LAIRD, DIANA J · 2010 to 2014
$2.4M
Signaling mechanisms that modulate uterine 3D structure for pregnancy successR01HD109152 · NICHD · MICHIGAN STATE UNIVERSITY · PI Ripla Arora · 2022 to 2026
$2.0M
Fmr1 Function and Repeat Expansion in the Developing GermlineR01GM122902 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LAIRD, DIANA J · 2018 to 2021
$1.8M
The intersection of stress and environmental chemicals in germ cell reprogrammingR01ES028212 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LAIRD, DIANA J · 2017 to 2021
$1.7M
Interrogating the Role of HSF1 in Ovarian StressF31HD110208 · NICHD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FOECKE, MARIKO · 2022 to 2024
$130k
NICHD NIH HHS F31 HD110208NICHD NIH HHS R01 HD109152NICHD NIH HHS T32 HD007470NIEHS NIH HHS P30 ES030284NIEHS NIH HHS R01 ES028212NIGMS NIH HHS R01 GM122902NIH HHS DP2 OD007420
6 · The paper itself

Abstract

Recent advances in tissue clearing methodologies have enabled three-dimensional (3D) visualization of the ovary and, consequently, in-depth exploration of the dynamic changes occurring at the single-cell level. Here we describe methods for whole-mount immunofluorescence, clearing, imaging, and analysis of whole ovarian tissue in 3D throughout murine development and aging.

Indexed as

Imaging, Three-DimensionalOvaryAgingAnimalsFemaleFluorescent Antibody TechniqueMice3D analysisImagingOvaryTissue clearingWhole-mount immunofluorescence

Identifiers

PMID37464244
PMCPMC10796149

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.