Evidence map›Paper›PMID 37464238›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Live Imaging of the Drosophila Testis Stem Cell Niche.

Leah J Greenspan, Erika L Matunis

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Leah J GreenspanDivision of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
Erika L MatunisDepartment of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. matunis@jhmi.edu.

Funding

BIOCHEMISTRY, CELLULAR AND MOLECULAR BIOLOGY PROGRAMT32GM007445 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI GREEN, RACHEL · 1985 to 2021
$24.1M
Cellular plasticity in the Drosophila spermatogonial stem cell nicheR01HD052937 · NICHD · JOHNS HOPKINS UNIVERSITY · PI MATUNIS, ERIKA L · 2007 to 2020
$3.8M
Zeiss LSM780 Confocal Microscope for a Core FacilityS10OD016374 · OD · JOHNS HOPKINS UNIVERSITY · PI KUO, SCOT CHARLES · 2013 to 2013
$584k
Regulation of niche cell plasticity in the testisF31HD085748 · NICHD · JOHNS HOPKINS UNIVERSITY · PI GREENSPAN, LEAH JOY · 2016 to 2017
$88k
NICHD NIH HHS F31 HD085748NICHD NIH HHS R01 HD052937NIGMS NIH HHS T32 GM007445NIH HHS S10 OD016374
6 · The paper itself

Abstract

Live imaging of adult tissue stem cell niches provides key insights into the dynamic behavior of stem cells, their differentiating progeny, and their neighboring support cells, but few niches are amenable to this approach. Here, we discuss a technique for long-term live imaging of the Drosophila testis stem cell niche. Culturing whole testes ex vivo for up to 18 h allows for tracking of cell-type-specific behaviors under normal and various chemically or genetically modified conditions. Fixing and staining tissues after live imaging allows for the molecular confirmation of cell identity and behavior. By using live imaging in intact niches, we can better uncover the cellular and molecular mechanisms that regulate stem cell function in vivo.

Indexed as

DrosophilaDrosophila ProteinsAnimalsDrosophila melanogasterMaleStem Cell NicheStem CellsTestisDrosophila ProteinsConfocal microscopyDrosophila testisFluorescenceNicheStem cellTime-lapse live-imaging

Identifiers

PMID37464238

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.