Evidence map›Paper›PMID 37464237›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Targeting Endogenous Loci That Function in Drosophila Germline Stem Cells.

Marianne Mercer, Varsha Bhargava, Courtney D Goldstein, Michael Buszczak

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Bourbon and Mycbp function with Otu to promote Sxl protein expression in theProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Marianne MercerDepartment of Molecular Biology, The University of Texas Southwestern Medical Center, Dallas, TX, USA.
Varsha BhargavaDepartment of Molecular Biology, The University of Texas Southwestern Medical Center, Dallas, TX, USA.
Courtney D GoldsteinDepartment of Molecular Biology, The University of Texas Southwestern Medical Center, Dallas, TX, USA.
Michael BuszczakDepartment of Molecular Biology, The University of Texas Southwestern Medical Center, Dallas, TX, USA. Michael.buszczak@utsouthwestern.edu.

Funding

Genetic Dissection of Germ Cell Differentiation and FunctionR35GM144043 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI Michael Buszczak · 2022 to 2026
$2.1M
Role of GCNA in preserving genome integrity and fertilityR01GM127569 · NIGMS · MAGEE-WOMEN'S RES INST AND FOUNDATION · PI BUSZCZAK, MICHAEL, YANOWITZ, JUDITH L · 2019 to 2022
$2.0M
NIGMS NIH HHS R01 GM127569NIGMS NIH HHS R35 GM144043
6 · The paper itself

Abstract

CRISPR-Cas9 genome editing technology can be used to manipulate the genome of Drosophila melanogaster. The ability to delete genes, make specific mutations, add tags, or make other genetic manipulations is useful for studying germline stem cell biology. In this chapter, we will describe a method to use CRISPR-Cas9 genome editing technology to make knock-out and knock-in flies. We will cover everything from guideRNA (gRNA) and donor plasmid design and cloning to screening for positive edits.

Indexed as

DrosophilaDrosophila melanogasterAnimalsCRISPR-Cas SystemsGerm CellsStem CellsCRISPR-Cas9Drosophila ovaryGenome editingGermline stem cells

Identifiers

PMID37464237

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.