Evidence map›Paper›PMID 37462278›Full record

ArticleG3 (Bethesda, Md.)2023

Demonstration of in vivo engineered tandem duplications of varying sizes using CRISPR and recombinases in Drosophila melanogaster.

David W Loehlin, Georgia L McClain, Manting Xu, Ria Kedia, Elise Root

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in G3 (Bethesda, Md.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

David W LoehlinBiology Department, Williams College, Williamstown, MA 01267, USA.
Georgia L McClainBiology Department, Williams College, Williamstown, MA 01267, USA.
Manting XuBiology Department, Williams College, Williamstown, MA 01267, USA.
Ria KediaBiology Department, Williams College, Williamstown, MA 01267, USA.
Elise RootBiology Department, Williams College, Williamstown, MA 01267, USA.
Williams College · US

Funding

Causes of excess expression of tandem duplicate genesR15GM140429 · NIGMS · WILLIAMS COLLEGE · PI LOEHLIN, DAVID WALKER · 2020 to 2020
$402k
NIGMS NIH HHS R15 GM140429
6 · The paper itself

Abstract

Tandem gene duplicates are important parts of eukaryotic genome structure, yet the phenotypic effects of new tandem duplications are not well-understood, in part owing to a lack of techniques to build and modify them. We introduce a method, Recombinase-Mediated Tandem Duplication, to engineer specific tandem duplications in vivo using CRISPR and recombinases. We describe construction of four different tandem duplications of the Alcohol Dehydrogenase (Adh) gene in Drosophila melanogaster, with duplicated block sizes ranging from 4.2 to 20.7 kb. Flies with the Adh duplications show elevated ADH enzyme activity over unduplicated single copies. This approach to engineering duplications is combinatoric, opening the door to systematic study of the relationship between the structure of tandem duplications and their effects on expression.

Indexed as

Drosophila melanogasterGene DuplicationAnimalsClustered Regularly Interspaced Short Palindromic RepeatsGenomeAdhAlcohol dehydrogenasecopy-number variationCRISPRgenome engineeringrecombinasesegmental duplicationtandem duplication

Identifiers

PMID37462278
PMCPMC10542505
OpenAlexW4384626031

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.