Evidence map›Paper›PMID 37461709›Full record

ArticleResearch square2023

Structure and dynamics of endogenous protein complexes in human heart tissue captured by native nanoproteomics.

Emily A Chapman, David S Roberts, Timothy N Tiambeng, Jãán Andrews, Man-Di Wang, Emily A Reasoner, Jake A Melby, Brad H Li, Donguk Kim, Andrew J Alpert and 2 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Emily A ChapmanDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.ORCID 0000-0003-1110-8984
David S RobertsDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.ORCID 0000-0002-0478-4987
Timothy N TiambengDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Jãán AndrewsDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Man-Di WangDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Emily A ReasonerDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Jake A MelbyDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Brad H LiDepartment of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Donguk KimDepartment of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Andrew J AlpertPolyLC Inc., Columbia, Maryland 21045, USA.
Song JinDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.ORCID 0000-0001-8693-7010
Ying GeDepartment of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.ORCID 0000-0001-5211-6812
University of Wisconsin–Madison · USPolytec (Germany) · DE

Funding

Training Program in Translational Cardiovascular Science (TPTCS)T32HL007936 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI Lee Lochbaum Eckhardt, Gail A Robertson · 2001 to 2026
$11.6M
Chemistry-Biology Interface Training ProgramT32GM008505 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI BLACKWELL, HELEN E. · 1993 to 2023
$9.8M
Deciphering Myofilament Modifications in Ischemic CardiomyopathyR01HL109810 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING · 2013 to 2024
$3.5M
Enabling Top-Down Proteomics through Materials Chemistry and NanotechnologyR01GM117058 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING, JIN, SONG · 2015 to 2023
$2.7M
Ultra High Resolution Mass Spectrometer for Biomedical ResearchS10OD018475 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING · 2015 to 2015
$2.0M
MASH Explorer, a Comprehensive Software Environment for Top-Down ProteomicsR01GM125085 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING · 2018 to 2021
$1.2M
NHLBI NIH HHS R01 HL109810NHLBI NIH HHS T32 HL007936NIGMS NIH HHS R01 GM117058NIGMS NIH HHS R01 GM125085NIGMS NIH HHS T32 GM008505NIH HHS S10 OD018475
6 · The paper itself

Abstract

Protein complexes are highly dynamic entities that display substantial diversity in their assembly, post-translational modifications, and non-covalent interactions, allowing them to play critical roles in various biological processes. The heterogeneity, dynamic nature, and low abundance of protein complexes in their native states present tremendous challenges to study using conventional structural biology techniques. Here we develop a "native nanoproteomics" strategy for the native enrichment and subsequent native top-down mass spectrometry (nTDMS) of low-abundance protein complexes. Specifically, we demonstrate the first comprehensive characterization of the structure and dynamics of cardiac troponin (cTn) complexes directly from human heart tissue. The endogenous cTn complex is effectively enriched and purified using peptide-functionalized superparamagnetic nanoparticles under non-denaturing conditions to enable the isotopic resolution of cTn complexes, revealing their complex structure and assembly. Moreover, nTDMS elucidates the stoichiometry and composition of the heterotrimeric cTn complex, localizes Ca

Identifiers

PMID37461709
PMCPMC10350235
OpenAlexW4383311880

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.