Evidence map›Paper›PMID 37461465›Full record

ArticlebioRxiv : the preprint server for biology2023

Synaptophysin Chaperones the Assembly of 12 SNAREpins under each Ready-Release Vesicle.

Manindra Bera, Abhijith Radhakrishnan, Jeff Coleman, Ramalingam Venkat Kalyana Sundaram, Sathish Ramakrishnan, Frederic Pincet, James E Rothman

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Manindra BeraNanobiology Institute, Yale University School of Medicine, New Haven, CT 06520, USA.
Abhijith RadhakrishnanNanobiology Institute, Yale University School of Medicine, New Haven, CT 06520, USA.
Jeff ColemanNanobiology Institute, Yale University School of Medicine, New Haven, CT 06520, USA.
Ramalingam Venkat Kalyana SundaramNanobiology Institute, Yale University School of Medicine, New Haven, CT 06520, USA.
Sathish RamakrishnanNanobiology Institute, Yale University School of Medicine, New Haven, CT 06520, USA.
Frederic PincetNanobiology Institute, Yale University School of Medicine, New Haven, CT 06520, USA.
James E RothmanNanobiology Institute, Yale University School of Medicine, New Haven, CT 06520, USA.
Yale University · USCentre National de la Recherche Scientifique · FR

Funding

Regulation of Membrane Fusion in ExocytosisR01DK027044 · NIDDK · YALE UNIVERSITY · PI KRISHNAKUMAR, SHYAM S · 1986 to 2021
$8.2M
MECHANISMS OF INTRACELLULAR MEMBRANE FUSIONR37DK027044 · NIDDK · YALE UNIVERSITY · PI ROTHMAN, JAMES · 1992 to 2012
$5.3M
NIDDK NIH HHS R01 DK027044NIDDK NIH HHS R37 DK027044
6 · The paper itself

Abstract

The synaptic vesicle protein Synaptophysin has long been known to form a complex with the v-SNARE VAMP, but a more specific molecular function or mechanism of action in exocytosis has been lacking because gene knockouts have minimal effects. Utilizing fully-defined reconstitution and single-molecule measurements, we now report that Synaptophysin functions as a chaperone that determines the number of SNAREpins assembling between a ready-release vesicle and its target membrane bilayer. Specifically, Synaptophysin directs the assembly of 12 ± 1 SNAREpins under each docked vesicle, even in the face of an excess of SNARE proteins. The SNAREpins assemble in successive waves of 6 ± 1 and 5 ± 2 SNAREpins, respectively, tightly linked to oligomerization of and binding to the vesicle Ca

Indexed as

Neurotransmitter releaseSNAREpinsSynaptophysinSynaptotagmin1VAMP2

Identifiers

PMID37461465
PMCPMC10349951
OpenAlexW4383370175

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.