Evidence map›Paper›PMID 37460950›Full record

ArticleBMC microbiology2023

Phenotypical characterization, and antibiotics susceptibility patterns of skin bacteria found in podoconiosis patients in the North West Region of Cameroon.

Derick Lekealem Nkwetta, Bangsi Rose Fuen, Njodzeka Flora Yenban, Nancielle Mbiatong, Gordon Takop Nchanji, John Bonekeh, Bertrand Lontum Ndzeshang, Narcisse Victor Tchamatchoua Gandjui, Fanny Fri Fombad, Ute Klarmann-Schulz and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
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Article in BMC microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 5 institutions in 3 countries.

Derick Lekealem NkwettaDepartment of Microbiology and Parasitology, University of Buea, P.O. Box 63, Buea, Cameroon.
Bangsi Rose FuenDepartment of Microbiology and Parasitology, University of Buea, P.O. Box 63, Buea, Cameroon.
Njodzeka Flora YenbanDepartment of Microbiology and Parasitology, University of Buea, P.O. Box 63, Buea, Cameroon.
Nancielle MbiatongDepartment of Microbiology and Parasitology, University of Buea, P.O. Box 63, Buea, Cameroon.
Gordon Takop NchanjiDepartment of Microbiology and Parasitology, University of Buea, P.O. Box 63, Buea, Cameroon.
John BonekehThe Peoples' Hope Medical Care Centre (PHOMECC), Bamenda, Cameroon.
Bertrand Lontum NdzeshangDepartment of Microbiology and Parasitology, University of Buea, P.O. Box 63, Buea, Cameroon.
Narcisse Victor Tchamatchoua GandjuiDepartment of Microbiology and Parasitology, University of Buea, P.O. Box 63, Buea, Cameroon.
Fanny Fri FombadDepartment of Microbiology and Parasitology, University of Buea, P.O. Box 63, Buea, Cameroon.
Ute Klarmann-SchulzInstitute for Medical Microbiology, Immunology and Parasitology (IMMIP), University Hospital Bonn (UKB), Bonn, Germany.
Mathias Eyong EsumDepartment of Microbiology and Parasitology, University of Buea, P.O. Box 63, Buea, Cameroon.
Abdel Jelil NjouendouResearch Foundation for Tropical Diseases and the Environment (REFOTDE), P.O. Box 474, Buea, Cameroon.
Jerome Fru ChoDepartment of Microbiology and Parasitology, University of Buea, P.O. Box 63, Buea, Cameroon.
Achim HoeraufInstitute for Medical Microbiology, Immunology and Parasitology (IMMIP), University Hospital Bonn (UKB), Bonn, Germany.
Manuel Ritter *Institute for Medical Microbiology, Immunology and Parasitology (IMMIP), University Hospital Bonn (UKB), Bonn, Germany.
Samuel Wanji *Department of Microbiology and Parasitology, University of Buea, P.O. Box 63, Buea, Cameroon. swanji@yahoo.fr.
University of Buea · CMUniversity of Bamenda · CMUniversity Hospital Bonn · DEGerman Center for Infection Research · DEYahoo (Spain) · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPodoconiosis, a non-infectious disease originating from long-term exposure of bare feet to irritant red clay soil is a lifelong, disabling disease with no specific diagnostic tool, classified into 5 stages based on the severity of leg swelling (lymphoedema). Secondary bacterial infections have been suggested to cause acute dermatolymphangioadenitis (ADLA) attacks and drive disease progression. Although the North West Region of Cameroon has a proven history of podoconiosis endemicity, the bacterial composition of lymphoedema due to this condition has not been studied. Thus, this study investigated the leg bacterial diversity of patients who suffered from the lymphoedema and their susceptibility pattern to selected antibiotics.

methodsA cross-sectional study was carried out in which podoconiosis affected and non-lymphoedema individuals living in the same community were purposively selected. Samples were collected by swabbing the skin between the toes and around the anklebone, then cultured and sub-cultured on nutrient agar to obtain pure isolates. The cultured isolates were then morphologically and biochemically classified using microscopy and analytic profile index test kits, respectively. The disk diffusion technique was used to determine antibiotic susceptibility.

resultsThirty-three participants were recruited, and 249 bacterial isolates were characterized into 29 genera, 60 species; with 30 (50%) being gram positive rods, 19 (31.7%) gram positive cocci, and 11 (18.3%) gram negative rods. Thirteen gram positive rods, fifteen gram positive cocci, and eight gram negative rods of bacterial species were found only in podoconiosis individuals among which Cellulomonas spp / Microbacterium spp. (2.8%), Staphylococcus lentus (3.3%), and Burkholderia cepacia (4.0%) dominated. 90% (90%) of the bacterial isolates were sensitive to doxycycline, whereas ampicillin had a high level of intermediate resistance, and penicillin G had the greatest resistant profile.

conclusionOur findings show that 94 (37.8%) out of 249 described bacterial isolates were exclusively found in the legs of podoconiosis individuals, and their susceptibility pattern to antibiotics was similar to that of others.

Indexed as

ElephantiasisLymphedemaAnti-Bacterial AgentsCameroonCross-Sectional StudiesGram-Negative BacteriaHumansMicrobial Sensitivity TestsAnti-Bacterial AgentsAntibioticbacteriaPhenotypicPodoconiosisSkinSusceptibility

Identifiers

PMID37460950
PMCPMC10351198
OpenAlexW4384557406

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.