ArticleCell research2023
Dissecting peri-implantation development using cultured human embryos and embryo-like assembloids.
Article in Cell research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed.
- Generation of eight-cell embryo-like cells from human pluripotent stem cells.Nature protocols · 2026Review
- Establishment of Human Formative Pluripotent Stem Cell-Like Cells Exhibiting Amniotic Differentiation Potentials.Cell proliferation · 2026Article
- Signaling mechanisms and dynamics governing the myocardial-epicardial fate switch during human cardiogenesis.Science advances · 2026Article
- Generating Microglia-Integrated Cortical Organoids from Human Embryonic Stem Cells.Stem cell reviews and reports · 2026Article
- Manipulating embryogenesis and testing for potential: two real problems for the regulation of stem cell-based embryo models.Journal of medical ethics · 2026Article
- A transgene-free, human peri-gastrulation embryo model presents trilaminar embryonic disc-, amnion- and yolk sac-like structures.Nature cell biology · 2026Article
- The vertebrate yolk sac: evolutionary origins and current advances in in vitro models.Development (Cambridge, England) · 2026Review
- Stem cell-based embryo models as a tool for reproductive biology.Molecular human reproduction · 2026Review
- Progress in stem cell-based embryo models and their applications in developmental biology and biomedicine.Nature reviews. Molecular cell biology · 2026Review
- Amniogenesis in embryos and stem cell models.Nature cell biology · 2026Review
- High resolution spatial transcriptomic and proteomic profiling of early primate gastrulationbioRxiv : the preprint server for biology · 2026Article
- Trophectoderm-like cells from EPS cells enable generating EPS cell-derived post-implantation embryoids that complete gastrulation.Protein & cell · 2026Article
- Modeling Post-Implantation Mammalian Embryogenesis Using AdvancedInternational journal of molecular sciences · 2026Review
- From Single Stem Cells to an In Vitro Model of the Post-implantation Human Embryo: A Step-by-Step Guide.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A human epiblast model reveals dynamic TGFβ-mediated control of epithelial identity during mammalian epiblast development.Nature cell biology · 2026Article
- Optogenetic Rescue Reveals Spatiotemporal Rules of Germ-Layer Patterning.bioRxiv : the preprint server for biology · 2025Article
- Review
- Establishment of human gastrulating stem cells with the capacity of stable differentiation into multiple gastrulating cell types.Cell research · 2025Article
- Stem cell-based human embryo models: current knowledge and open questions.Stem cell research & therapy · 2025Review
- Single Cell RNA Sequencing and Its Impact on Understanding Human Embryo Development.International journal of molecular sciences · 2025Review
Corrections and comments
- Commented on by
Authors and funding
25 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studies of cultured embryos have provided insights into human peri-implantation development. However, detailed knowledge of peri-implantation lineage development as well as underlying mechanisms remains obscure. Using 3D-cultured human embryos, herein we report a complete cell atlas of the early post-implantation lineages and decipher cellular composition and gene signatures of the epiblast and hypoblast derivatives. In addition, we develop an embryo-like assembloid (E-assembloid) by assembling naive hESCs and extraembryonic cells. Using human embryos and E-assembloids, we reveal that WNT, BMP and Nodal signaling pathways synergistically, but functionally differently, orchestrate human peri-implantation lineage development. Specially, we dissect mechanisms underlying extraembryonic mesoderm and extraembryonic endoderm specifications. Finally, an improved E-assembloid is developed to recapitulate the epiblast and hypoblast development and tissue architectures in the pre-gastrulation human embryo. Our findings provide insights into human peri-implantation development, and the E-assembloid offers a useful model to disentangle cellular behaviors and signaling interactions that drive human embryogenesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.