Evidence map›Paper›PMID 37458872›Full record

ArticleMolecular biology reports2023

A survey of frequency of virulence and aminoglycoside antibiotic-resistant genotypes and phenotypes in Escherichia coli in broilers in Khartoum State, Sudan.

Egbal Munir, Khalda Khalifa, Amgad Abdel Rahman, Eman Bakri, Khalid Rudwan, Mohammed Hussien

Abstract read
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In one paragraph

Article in Molecular biology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Egbal MunirCentral Veterinary Research Laboratory (CVRL), Animal Resources Research Corporation (ARRC), El Amarat, P.O. Box 8067, Khartoum, Sudan.
Khalda KhalifaCentral Veterinary Research Laboratory (CVRL), Animal Resources Research Corporation (ARRC), El Amarat, P.O. Box 8067, Khartoum, Sudan.
Amgad Abdel RahmanCentral Veterinary Research Laboratory (CVRL), Animal Resources Research Corporation (ARRC), El Amarat, P.O. Box 8067, Khartoum, Sudan.
Eman BakriCentral laboratory, Ministry of Higher Education and Scientific Research, P.O. Box 7099, Khartoum, Sudan.
Khalid RudwanSudan University of Science and Technology, Ministry of Higher Education and Scientific Research, P.O. Box 2081, Khartoum, Sudan.
Mohammed HussienCentral laboratory, Ministry of Higher Education and Scientific Research, P.O. Box 7099, Khartoum, Sudan. moh200432@hotmail.com.ORCID http://orcid.org/0000-0002-6661-2988
Agricultural Research Corporation · SDMinistry of Higher Education And Scientific Research · SD

Funding

Central Veterinary Research Laboratory, ARRC Central Veterinary Research Laboratory, ARRC
6 · The paper itself

Abstract

backgroundAlthough Escherichia coli (E. coli) is considered a normal microflora in the poultry intestine, certain strains namely, Avian Pathogenic E. coli (APEC), cause colisepticaemia (fatal disease) in poultry. The aim of this study was to determine the prevalence of the virulence genes, i.e. (iroN, ompT, iss, iutA, and hlyF) and aminoglycoside-modifying enzyme (AME) genes, i.e. (strA and strB) in Escherichia coli strains in broilers in Khartoum State. METHODS AND

resultsA total of 25 E. coli isolates were collected from broilers farms. All isolates were screened for antimicrobial susceptibility tests using Kirby-Bauer disc diffusion method. In addition, all isolates were tested for the presence of virulence genes and modifying enzyme genes using the polymerase chain reaction (PCR). The results showed that the prevalence of positive strains to virulence genes were 14 (56%), 21 (84%), 14 (56%), 0 (0%) and 0 (0%) to iroN, iutA, hlyF, ompT and iss, respectively. Combined virulence genes include iroN, hlyF and iutA were detected in 14 (56%). The rates of resistance were as follows: Gentamycin: (32%), Kanamycin: (20%) and Streptomycin (16%). Of the genes tested, strA (72%) was the most commonly recognized gene followed by strB (56%).

conclusionsIt could be concluded that this is the first report of molecular survey of virulence and aminoglycoside-modifying enzyme (AME) resistant genes in APEC isolates from broiler in Sudan. Therefore, prohibition of non-curative application of antibiotic, dishearten their abuse and to be frequently observant by suppling suitable research-based policy for the poultry industry is warranted.

Indexed as

Escherichia coli InfectionsPoultry DiseasesAnimalsAnti-Bacterial AgentsChickensEscherichia coliGenotypePhenotypeStreptomycinSudanVirulenceAnti-Bacterial AgentsStreptomycinAminoglycoside-modifying enzymeBroilersE. coliSudanVirulence

Identifiers

PMID37458872
OpenAlexW4384523164

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.