Evidence map›Paper›PMID 37457919›Full record

ArticleAnalytical methods : advancing methods and applications2016

A disposable chemical heater and dry enzyme preparation for lysis and extraction of DNA and RNA from microorganisms.

J R Buser, X Zhang, S A Byrnes, P D Ladd, E K Heiniger, M D Wheeler, J D Bishop, J A Englund, B Lutz, B H Weigl and 1 more

Open access · hybridAbstract read
In one paragraph

Article in Analytical methods : advancing methods and applications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
4.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 35 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Micro and Nanoscale Technologies for Diagnosis of Viral Infections.Small (Weinheim an der Bergstrasse, Germany) · 2021
    Review
  10. Review
  11. Article
  12. Review
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

J R BuserBox 355061, Department of Bioengineering, University of Washington, Seattle, WA, United States of America.
X ZhangBox 355061, Department of Bioengineering, University of Washington, Seattle, WA, United States of America.
S A ByrnesBox 355061, Department of Bioengineering, University of Washington, Seattle, WA, United States of America.
P D LaddBox 355061, Department of Bioengineering, University of Washington, Seattle, WA, United States of America.
E K HeinigerBox 355061, Department of Bioengineering, University of Washington, Seattle, WA, United States of America.
M D WheelerBox 355061, Department of Bioengineering, University of Washington, Seattle, WA, United States of America.
J D BishopBox 355061, Department of Bioengineering, University of Washington, Seattle, WA, United States of America.
J A EnglundProgram in Infectious Diseases, Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America.
B LutzBox 355061, Department of Bioengineering, University of Washington, Seattle, WA, United States of America.
B H WeiglPATH, Seattle, WA, United States of America.
P YagerBox 355061, Department of Bioengineering, University of Washington, Seattle, WA, United States of America.
University of Washington · USProgram for Appropriate Technology in Health · USSeattle Children's Hospital · US

Funding

A high-sensitivity low-cost multiplexed immunoassay platform based on 2-dimensionR01AI096184 · NIAID · UNIVERSITY OF WASHINGTON · PI YAGER, PAUL · 2011 to 2015
$5.2M
HIVAcute: rapid sample-to-result self-testing for HIV RNAR33AI147354 · NIAID · UNIVERSITY OF WASHINGTON · PI YAGER, PAUL · 2022 to 2023
$1.5M
NIAID NIH HHS R01 AI096184NIAID NIH HHS R33 AI147354
6 · The paper itself

Abstract

Sample preparation, including bacterial lysis, remains a hurdle in the realization of complete point-of-care tests for many pathogens. Here, we developed a sample preparation methodology for enzymatic lysis and sample heating for low-resource, point-of-care applications. We show an instrument-free chemical heater system for rapid lysis of a gram-positive bacterium (

Identifiers

PMID37457919
PMCPMC10348399
OpenAlexW2290406427

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.