Evidence map›Paper›PMID 37456510›Full record

ArticleBioinformatics advances2023

TraceTrack, an open-source software for batch processing, alignment and visualization of sanger sequencing chromatograms.

Kveta Brazdilova, David Prihoda, Quynh Ton, Heath Klock, Danny A Bitton

Abstract read
In one paragraph

Article in Bioinformatics advances, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kveta BrazdilovaDiscovery Informatics, MSD Czech Republic s.r.o, Prague 150 00, Czech Republic.
David PrihodaDiscovery Informatics, MSD Czech Republic s.r.o, Prague 150 00, Czech Republic.
Quynh TonProtein Sciences, MRL, Merck & Co., Inc., Boston, MA 02115, USA.
Heath KlockProtein Sciences, MRL, Merck & Co., Inc., Boston, MA 02115, USA.
Danny A BittonDiscovery Informatics, MSD Czech Republic s.r.o, Prague 150 00, Czech Republic.ORCID https://orcid.org/0000-0003-3282-1017

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Motivation: Despite the advent of next-generation sequencing technology and its widespread applications, Sanger sequencing remains instrumental for molecular biology subcloning work in biological and medical research and indispensable for drug discovery campaigns. Although Sanger sequencing technology has been long established, existing software for processing and visualization of trace file chromatograms is limited in terms of functionality, scalability and availability for commercial use. Results: To fill this gap, we developed TraceTrack, an open-source web application tool for batch alignment, analysis and visualization of Sanger trace files. TraceTrack offers high-throughput matching of trace files to reference sequences, rapid identification of mutations and an intuitive chromatogram analysis. Comparative analysis between TraceTrack and existing software tools highlights the advantages of TraceTrack with regards to batch processing, visualization and export functionalities. Availability and implementation: TraceTrack is available at https://github.com/MSDLLCpapers/TraceTrack and as a web application at https://tracetrack.dichlab.org. TraceTrack is a web application for batch processing and visualization of Sanger trace file chromatograms that meets the increasing demand of industrial sequence validation workflows in pharmaceutical settings. Supplementary information: Supplementary data are available at

Identifiers

PMID37456510
PMCPMC10348866

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.