Evidence map›Paper›PMID 37454765›Full record

ArticleSLAS technology2023

Automated human induced pluripotent stem cell colony segmentation for use in cell culture automation applications.

Kimerly A Powell, Laura R Bohrer, Nicholas E Stone, Bradley Hittle, Kristin R Anfinson, Viviane Luangphakdy, George Muschler, Robert F Mullins, Edwin M Stone, Budd A Tucker

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in SLAS technology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Advancements in theFrontiers in toxicology · 2025
    Review
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kimerly A PowellDepartment of Biomedical Informatics, The Ohio State University, Columbus, OH 43210, USA. Electronic address: kimerly.powell@osumc.edu.
Laura R BohrerInstitute for Vision Research, Carver College of Medicine, University of Iowa, Iowa City, IA, USA; Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Nicholas E StoneInstitute for Vision Research, Carver College of Medicine, University of Iowa, Iowa City, IA, USA; Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Bradley HittleDepartment of Biomedical Informatics, The Ohio State University, Columbus, OH 43210, USA.
Kristin R AnfinsonInstitute for Vision Research, Carver College of Medicine, University of Iowa, Iowa City, IA, USA; Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Viviane LuangphakdyDepartment of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA; Cell X Technologies Inc., Cleveland, OH, USA.
George MuschlerDepartment of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA; Department of Orthopedic Surgery, Cleveland Clinic, Cleveland, OH, USA.
Robert F MullinsInstitute for Vision Research, Carver College of Medicine, University of Iowa, Iowa City, IA, USA; Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Edwin M StoneInstitute for Vision Research, Carver College of Medicine, University of Iowa, Iowa City, IA, USA; Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.
Budd A TuckerInstitute for Vision Research, Carver College of Medicine, University of Iowa, Iowa City, IA, USA; Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.

Funding

Understanding and Using Variation in Source Materials for MSC FabricationR01DE029634 · NIDCR · CLEVELAND CLINIC LERNER COM-CWRU · PI MUSCHLER, GEORGE F · 2019 to 2023
$3.1M
A Next Generation Outer Retinal Microphysiological SystemR01EY033331 · NEI · UNIVERSITY OF IOWA · PI MULLINS, ROBERT FOSTER, STONE, EDWIN M · 2022 to 2025
$1.5M
Automation and Performance-Based Selection to Improve the Reproducibility of MSCsR44GM133291 · NIGMS · CELL X TECHNOLOGIES, INC. · PI LUANGPHAKDY, VIVIANE · 2019 to 2021
$1.4M
NEI NIH HHS R01 EY033331NIDCR NIH HHS R01 DE029634NIGMS NIH HHS R44 GM133291
6 · The paper itself

Abstract

Human induced pluripotent stem cells (hiPSCs) have demonstrated great promise for a variety of applications that include cell therapy and regenerative medicine. Production of clinical grade hiPSCs requires reproducible manufacturing methods with stringent quality-controls such as those provided by image-controlled robotic processing systems. In this paper we present an automated image analysis method for identifying and picking hiPSC colonies for clonal expansion using the CellX

Indexed as

Induced Pluripotent Stem CellsAutomationCell Culture TechniquesCell DifferentiationHumansRegenerative MedicineAutomated image analysis cell culturesDeep learningHuman induced pluripotent stem cell (hiPSC) processingStem cell manufacturing

Identifiers

PMID37454765
PMCPMC10775697

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.