ArticleThe Journal of cell biology1986
Studies on the transcription, translation, and structure of alpha-actinin in Dictyostelium discoideum.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 46 citations in OpenAlex.
- Phylogenetic analysis of gene structure and alternative splicing in alpha-actinins.Molecular biology and evolution · 2010Article
- DdLIM is a cytoskeleton-associated protein involved in the protrusion of lamellipodia in Dictyostelium.Molecular biology of the cell · 1998Article
- The Dictyostelium cytoskeleton.Experientia · 1995Review
- Invariant tryptophan at a shielded site promotes folding of the conformational unit of spectrin.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Evidence for a feedback regulated back-up promoter which controls permanent expression of a Dictyostelium gene.Nucleic acids research · 1990Article
- Constitutive overexpression of the contact site A glycoprotein enables growth-phase cells of Dictyostelium discoideum to aggregate.The EMBO journal · 1990Article
- The extrachromosomal replication of Dictyostelium plasmid Ddp2 requires a cis-acting element and a plasmid-encoded trans-acting factor.Molecular and cellular biology · 1990Article
- Alpha-actinin synthesis can be modulated by antisense probes and is autoregulated in non-muscle cells.The EMBO journal · 1989Article
- The cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum utilizes alternate promoters and splicing for the synthesis of multiple mRNAs.Molecular and cellular biology · 1989Article
- The Dictyostelium gelation factor shares a putative actin binding site with alpha-actinins and dystrophin and also has a rod domain containing six 100-residue motifs that appear to have a cross-beta conformation.The Journal of cell biology · 1989Article
- Linkage analysis of the myosin heavy chain gene in Dictyostelium discoideum using a mutation generated by homologous recombination.Molecular & general genetics : MGG · 1989Article
- Codon preference in Dictyostelium discoideum.Nucleic acids research · 1988Article
- Article
- Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein.The EMBO journal · 1987Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A clone coding for the F-actin cross-linking protein alpha-actinin was obtained by screening a genomic library of Dictyostelium discoideum DNA in lambda gt11 with monoclonal antibodies specific for Dictyostelium alpha-actinin. The 1.2-kilobase (kb) genomic clone was confirmed as containing part of the alpha-actinin gene by comparing its nucleotide sequence with the amino acid sequence of tryptic peptides from purified alpha-actinin. The clone recognized a 3.0-kb message in a Northern blot. Hybridization to RNA isolated from different developmental stages of several D. discoideum strains indicated that the mRNA content increased during early development. A similar result was obtained when the alpha-actinin content of the cells was followed by Western blot analysis. Hybridization of the clone to DNA from different wild-type strains of D. discoideum indicated a polymorphism on the DNA level that coincided with a polymorphism on the protein level. The data suggest continuous transcription of the alpha-actinin gene throughout the development of D. discoideum, up- and down-regulation of the levels of alpha-actinin mRNA and protein with maximum levels at the onset of aggregation, and a high diversity of alpha-actinin at the DNA and protein level among different D. discoideum strains. The structural data make it conceivable that the highly conserved nature of alpha-actinin resides only at the functional sites, whereas the helical portions of the alpha-actinin molecule allow a higher level of diversity throughout evolution.
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