ArticleScientific reports2023
Molecular detection of human Plasmodium species using a multiplex real time PCR.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Mechanistic insights into subclinicaliScience · 2026Review
- Accurate and sensitive TaqMan real-time PCR assays to detect and quantify simian malaria parasites Plasmodium cynomolgi, Plasmodium inui and Plasmodium coatneyi.Malaria journal · 2026Article
- Characterisation of malaria and glucose-6-phosphate dehydrogenase deficiency in conflict-affected zones of southern and eastern Sudan.BMC infectious diseases · 2026Article
- Current and emerging molecular diagnostic approaches in the detection of human parasites.Parasitology research · 2026Review
- FedMal-XAI: an explainable federated vision transformer leveraging knowledge distillation for privacy-preserving malaria detection.Frontiers in public health · 2026Article
- Fractal fractional approach to investigate the forecasting and dynamics of malaria disease for early control precaution.Scientific reports · 2025Article
- Sensitive near point-of-care detection of asymptomatic and submicroscopic Plasmodium falciparum infections in African endemic countries.Nature communications · 2025Article
- Molecular and Microscopic Challenges in DetectingPathogens (Basel, Switzerland) · 2025Article
- Breaking down malaria outbreak: A multidisciplinary approach in a border village of French Guiana.PLoS neglected tropical diseases · 2025Article
- Hematology thin smears perform equally to parasitology thick and thin blood smears for the diagnosis ofJournal of clinical microbiology · 2025Article
- Off-grid field-deployable molecular diagnostic platform for malaria surveillance.Parasites & vectors · 2025Article
- Article
- Plasmodium ovale Malaria in Travelers and Immigrants to the United States: A Case Series.The American journal of tropical medicine and hygiene · 2025Article
- Evaluation of Two Multiplexed qPCR Assays for Malaria Detection and Speciation: A Comparative Study With Nested PCR and Microscopy.Journal of parasitology research · 2025Article
- Identification of visible and near-infrared signature peaks for arboviruses and Plasmodium falciparum.PloS one · 2025Article
- Spatiotemporal dynamics of malaria and climate influence on its incidence in Condorcanqui Province, 2005-2022.Malaria journal · 2024Article
- Which diagnostic test to use for Testing and Treatment strategies inLancet regional health. Americas · 2024Article
- Asymptomatic Malaria Cases andPathogens (Basel, Switzerland) · 2024Article
- Clinical significance of PLT for diagnosis and treatment monitoring in imported malaria.Scientific reports · 2024Article
- Modern Strategies for Diagnosis and Treatment of Parasitic Diseases.International journal of molecular sciences · 2024Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular detection methods have revealed higher sensitivity and specificity than conventional microscopy or rapid diagnostic tests for malaria diagnosis. In this study, we implemented, evaluated and validated according to the ISO 15,189 requirements, a multiplex real-time PCR assay to detect and identify the five human malaria parasites. DNA samples were extracted from whole blood or dried blood spots drawn from patients. Based on the External Quality Assessment (whole blood), this method shows 100% sensitivity and specificity. This PCR detected P. vivax up to 0.25 p/µl, P. falciparum and P. knowlesi up to 0.5 p/µl, P. ovale up to 1 p/µl and P. malariae up to 5 p/µl of blood. From blood spots (extraction from four punches), it detected P. vivax at 5 p/µl, P. falciparum, P. ovale and P. knowlesi at 20 p/µl and P. malariae at 125 p/µl. In conclusion, this quantitative PCR shows excellent performance, is easy to use and DNA saver. It is especially useful to actively screen large population groups and identify the five human malaria parasites in a context of low malaria transmission.
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