Evidence map›Paper›PMID 37450150›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Next-Generation Yeast Two-Hybrid Screening to Discover Protein-Protein Interactions.

J Mitch Elmore, Valeria Velásquez-Zapata, Roger P Wise

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Powdery mildew effectors AVRMolecular plant pathology · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

J Mitch ElmoreUSDA-Agricultural Research Service, Cereal Disease Laboratory, St. Paul, MN, USA. mitch.elmore@usda.gov.
Valeria Velásquez-ZapataDepartment of Plant Pathology, Entomology and Microbiology, Iowa State University, Ames, IA, USA.
Roger P WiseUSDA-Agricultural Research Service, Corn Insects and Crop Genetics Research, Ames, IA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Yeast two-hybrid is a powerful approach to discover new protein-protein interactions. Traditional methods involve screening a target protein against a cDNA expression library and assaying individual positive colonies to identify interacting partners. Here we describe a simple approach to perform yeast two-hybrid screens of a cDNA expression library in batch liquid culture. Positive yeast cell populations are enriched under selection and then harvested en masse. Prey cDNAs are amplified and used as input for next-generation sequencing libraries for identification, quantification, and ranking.

Indexed as

Saccharomyces cerevisiaeDNA, ComplementaryGene LibraryTwo-Hybrid System TechniquesDNA, ComplementarycDNA libraryInteraction screeningProtein–protein interactionsSequencingYeast two-hybrid

Identifiers

PMID37450150

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.