ArticleMethods in molecular biology (Clifton, N.J.)2023
Detection of Protein-Protein Interactions Utilizing the Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 3 citations in OpenAlex.
- Screening and functional analysis of a host membrane protein interacting with the structural protein VP1 of deformed wing virus.Frontiers in microbiology · 2026Article
- The Use of Yeast Two-Hybrid Analysis to Study Protein-Protein Interactions for Ustilago maydis.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Identification of Virus-Host Interactions.Advances in experimental medicine and biology · 2026Review
- SlATG8f coordinates ethylene signaling and chloroplast turnover to drive tomato fruit ripening.Plant cell reports · 2025Article
- Construction of a Membrane Yeast Two-Hybrid Library and Screening of MsPYR1-Like Interacting Proteins in Malus sieversii.Molecular biotechnology · 2025Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Identifying the interactors of a protein is a key step in understanding its possible cellular function(s). Among the various methods that can be used to study protein-protein interactions (PPIs), the yeast two-hybrid (Y2H) assay is one of the most standardized, sensitive, and cost-effective in vivo methods available. The most commonly used GAL4-based Y2H system utilizes the yeast transcription factor GAL4 to detect interactions between soluble proteins. By virtue of involving a transcription factor, the protein-protein interactions occur in the nucleus. The split-ubiquitin Y2H system offers an alternative to the traditional GAL4-based Y2H system and takes advantage of the reconstitution of split-ubiquitin in the cytosol to identify interactions between two proteins. Moreover, new membranous and soluble interacting partner(s) can be identified by screening a target protein against proteins produced from a cDNA library using this system.
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Registered trials
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