ArticleMethods in molecular biology (Clifton, N.J.)2023
Analyzing Protein-Protein Interactions Using the Split-Ubiquitin System.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- PAR2-β-arrestin 2-ERK axis mediatesiScience · 2026Article
- SNMPs Function as Molecular Bridges between OBPs and ORs in the Olfactory Recognition of Clostera Retitura (Walker) (Lepidoptera: Notodontidae).Journal of chemical ecology · 2025Article
- Large-scale protein interactome reveals lineage-specific genes driving plant-parasitic nematode adaptive innovations.Science advances · 2025Article
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Authors and funding
2 authors.
Funding
Abstract
The split-ubiquitin technology was developed over 20 years ago as an alternative to Gal4-based, yeast-two-hybrid methods to identify interacting protein partners. With the introduction of mating-based methods for split-ubiquitin screens, the approach has gained broad popularity because of its exceptionally high transformation efficiency, utility in working with full-length membrane proteins, and positive selection with little interference from spurious interactions. Recent advances now extend these split-ubiquitin methods to the analysis of interactions between otherwise soluble proteins and tripartite protein interactions.
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Registered trials
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