Evidence map›Paper›PMID 37450074›Full record

ArticleInflammopharmacology2023

Inhibition of CD4 + T cells by fanchinoline via miR506-3p/NFATc1 in Sjögren's syndrome.

Yanxiong Shao, Jiayao Fu, Tianle Zhan, Junhao Yin, Jiabao Xu, Yifan Lu, Qi Luo, Chuangqi Yu

Abstract read
PubMed Publisher
In one paragraph

Article in Inflammopharmacology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. The Role of Deregulated MicroRNAs in Immune Cells of Sjögren's Disease.International journal of general medicine · 2025
    Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Yanxiong Shao *Department of Oral Surgery, Shanghai Ninth People's HospitalCollege of Stomatology, National Center for Stomatology, National Clinical Research Center of Oral Disease, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University, Zhizaoju Road 639, Shanghai, 200011, China.
Jiayao Fu *Department of Oral Surgery, Shanghai Ninth People's HospitalCollege of Stomatology, National Center for Stomatology, National Clinical Research Center of Oral Disease, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University, Zhizaoju Road 639, Shanghai, 200011, China.
Tianle ZhanDepartment of Oral Surgery, Shanghai Ninth People's HospitalCollege of Stomatology, National Center for Stomatology, National Clinical Research Center of Oral Disease, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University, Zhizaoju Road 639, Shanghai, 200011, China.
Junhao YinDepartment of Oral Surgery, Shanghai Ninth People's HospitalCollege of Stomatology, National Center for Stomatology, National Clinical Research Center of Oral Disease, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University, Zhizaoju Road 639, Shanghai, 200011, China.
Jiabao XuDepartment of Oral Surgery, Shanghai Ninth People's HospitalCollege of Stomatology, National Center for Stomatology, National Clinical Research Center of Oral Disease, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University, Zhizaoju Road 639, Shanghai, 200011, China.
Yifan LuDepartment of Oral Surgery, Shanghai Ninth People's HospitalCollege of Stomatology, National Center for Stomatology, National Clinical Research Center of Oral Disease, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University, Zhizaoju Road 639, Shanghai, 200011, China.
Qi LuoDepartment of Oral Surgery, Shanghai Ninth People's HospitalCollege of Stomatology, National Center for Stomatology, National Clinical Research Center of Oral Disease, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University, Zhizaoju Road 639, Shanghai, 200011, China.
Chuangqi YuDepartment of Oral Surgery, Shanghai Ninth People's HospitalCollege of Stomatology, National Center for Stomatology, National Clinical Research Center of Oral Disease, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University, Zhizaoju Road 639, Shanghai, 200011, China. yuchuangqi2017@163.com.ORCID http://orcid.org/0000-0001-7929-5195
Shanghai Jiao Tong University · CN

Funding

Biological sample bank project of Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine YBKB201907National Natural Science Foundation of China 81771089
6 · The paper itself

Abstract

The hyperproliferation and hyperactivation of CD4 + T cells in salivary gland tissues are hallmarks of Sjögren's syndrome (SS). Fangchinoline (Fan) is extracted from the root of Stephania tetrandra Moore, which is used for treating rheumatic diseases in many studies. This study aimed to identify the mechanism underlying the inhibition of CD4 + T cells by Fan in the SS model NOD/ShiLtj mice. In vivo, Fan alleviated the dry mouth and lymphocyte infiltration in the salivary gland tissues of the NOD/ShiLtj mice and inhibited the number of CD4 + T cells in the infiltrating focus. In vitro, Fan's inhibitory effect on the proliferation of mouse primary CD4 + T cells was verified by CFSE and EdU tests. Furthermore, qRT-PCR and WB analysis confirmed that Fan could inhibit the expression of NFATc1 (Nuclear factor of activated T-cells, cytoplasmic 1) by upregulating miR-506-3p. Dual luciferase reporter gene assay suggested that miR-506-3p interacted with NFATc1. CFSE and EdU tests showed that Fan could inhibit the proliferation of CD4 + T cells through miR-506-3p/NFATc1. The key role of NFATc1 in the activation of CD4 + T cells and the high expression of NFATc1 in samples from SS patients suggested that NFATc1 might become a therapeutic target for SS. In vivo, 11R-VIVIT (NFATc1 inhibitor) alleviated SS-like symptoms. This study not only explained the new mechanism of Fan inhibiting proliferation of CD4 + T cells and alleviating SS-like symptoms but also provided NFATc1 as a potential target for the subsequent research and treatment of SS.

Indexed as

MicroRNAsSjogren's SyndromeAnimalsCD4-Positive T-LymphocytesDisease Models, AnimalFluoresceinsHumansMiceMice, Inbred NODSalivary GlandsSuccinimides5-(6)-carboxyfluorescein diacetate succinimidyl esterFluoresceinsMicroRNAsMIRN506 microRNA, humanSuccinimidesCD4 + T cellFangchinolinemiR-506-3pNFATc1Sjögren’s syndrome

Identifiers

PMID37450074
OpenAlexW4384340028

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.