ArticleHistochemistry1986
Ultrastructural localization of calcium in the CNS of vertebrates.
Article in Histochemistry, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 52 citations in OpenAlex.
- Osteopontin mediates the formation of corpora amylacea-like structures from degenerating neurons in the CA1 region of the rat hippocampus after ischemia.Cell and tissue research · 2022Article
- Spatiotemporal expression of osteopontin in the striatum of rats subjected to the mitochondrial toxin 3-nitropropionic acid correlates with microcalcification.Scientific reports · 2017Article
- Article
- Talampanel reduces the level of motoneuronal calcium in transgenic mutant SOD1 mice only if applied presymptomatically.Amyotrophic lateral sclerosis : official publication of the World Federation of Neurology Research Group on Motor Neuron Diseases · 2011Article
- Progressive secondary neurodegeneration and microcalcification co-occur in osteopontin-deficient mice.The American journal of pathology · 2010Article
- Depletion of calcium in the synaptic cleft of a calyx-type synapse in the rat brainstem.The Journal of physiology · 1999Article
- High resolution ultrastructural mapping of total calcium: electron spectroscopic imaging/electron energy loss spectroscopy analysis of a physically/chemically processed nerve-muscle preparation.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Calcium localization in the shell-forming tissue of the freshwater snail, Biomphalaria glabrata: a comparative study of various methods for localizing calcium.The Histochemical journal · 1992Article
- The distribution of calcium in undecalcified bone as revealed by an improved pyro-antimonate method.The Histochemical journal · 1992Article
- Ibotenic acid-induced calcium deposits in rat substantia nigra. Ultrastructure of their time-dependent formation.Acta neuropathologica · 1992Article
- Cortical alveoli of Paramecium: a vast submembranous calcium storage compartment.The Journal of cell biology · 1991Article
- Ultrastructural localization of endogenous calcium in the teleost retina.The Histochemical journal · 1990Article
- Ultrastructural distribution of Ca++ within neurons. An oxalate pyroantimonate study.Histochemistry · 1987Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ultrastructural localization of calcium in synaptic areas of the CNS of fish was investigated. Prefixation with phosphate-buffered glutaraldehyde followed by post-fixation with osmium/potassium-bichromate was used to precipitate and visualize endogenous calcium without the addition of external calcium. The presence of calcium in the electron-dense precipitates produced using this method was demonstrated by electron spectroscopic imaging using a Zeiss EM-902 transmission electron microscope, and in various control experiments using the calcium chelator EGTA. In the optic tectum of fish, electron dense precipitates containing calcium were found not only in intracellular compartments, e.g. the smooth endoplasmic reticulum, mitochondria and synaptic vesicles, but also at extracellular locations, particularly in synaptic clefts. In the extracellular sites, only chelate complexes of ionic calcium were found. This would seem to be in agreement with electrophysiological and biochemical data reported in earlier studies. Thus, using the present method, it should be possible to obtain further ultrastructural information concerning the mechanisms of synaptic transmission.
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