Evidence map›Paper›PMID 37446341›Full record

ArticleInternational journal of molecular sciences2023

Comparison of Four Methods of RNA Extraction and cDNA Synthesis from The Venom of Peruvian Snakes of the Genus

Daniel Torrejón, Javier Cárdenas, Diana Juárez, Jordano Espinoza, Alex Proleón, Andrés Agurto-Arteaga, Fanny Lazo, Mariana Leguía, Félix A Urra, Eladio F Sánchez and 3 more

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.6field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 6 institutions in 3 countries.

Daniel TorrejónLaboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Av. Venezuela Cdra 34 S/N, Ciudad Universitaria, Lima Cercado, Lima 15081, Peru.ORCID 0000-0001-9385-6921
Javier CárdenasLaboratorio de Bioquímica, Facultad de Ciencias de la Salud, Universidad Nacional del del Callao, Av. Juan Pablo ΙΙ 306, Bellavista 07011, Peru.ORCID 0000-0002-6112-2584
Diana JuárezLaboratorio de Genómica, Pontificia Universidad Católica del Perú, Av. Universitaria 1801, Campus Principal, San Miguel 15088, Peru.
Jordano EspinozaLaboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Av. Venezuela Cdra 34 S/N, Ciudad Universitaria, Lima Cercado, Lima 15081, Peru.
Alex ProleónLaboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Av. Venezuela Cdra 34 S/N, Ciudad Universitaria, Lima Cercado, Lima 15081, Peru.
Andrés Agurto-ArteagaLaboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Av. Venezuela Cdra 34 S/N, Ciudad Universitaria, Lima Cercado, Lima 15081, Peru.
Fanny LazoLaboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Av. Venezuela Cdra 34 S/N, Ciudad Universitaria, Lima Cercado, Lima 15081, Peru.
Mariana LeguíaLaboratorio de Genómica, Pontificia Universidad Católica del Perú, Av. Universitaria 1801, Campus Principal, San Miguel 15088, Peru.ORCID 0000-0002-7297-0263
Félix A UrraLaboratorio de Plasticidad Metabólica y Bioenergética, Programa de Farmacología Clínica y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile.ORCID 0000-0001-8582-4399
Eladio F SánchezNetwork for Snake Venom Research and Drug Discovery, Av. Independencia 1027, Santiago 7810000, Chile.
Carlos Chávez-OlorteguiDepartamento de Bioquímica-Inmunología, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
Dan E Vivas-RuizLaboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Av. Venezuela Cdra 34 S/N, Ciudad Universitaria, Lima Cercado, Lima 15081, Peru.ORCID 0000-0003-4417-2026
Armando YarlequéLaboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Av. Venezuela Cdra 34 S/N, Ciudad Universitaria, Lima Cercado, Lima 15081, Peru.
National University of San Marcos · PEPontificia Universidad Católica del Perú · PEEzequiel Dias Foundation · BRUniversidade Federal de Minas Gerais · BRUniversidad Nacional del Callao · PEUniversity of Chile · CL

Funding

Programa Nacional de Investigación Científica y Estudios Avanzados, PROCIENCIA N° 079 - 2021 - FONDECYT and N° PE501080181-2022-PROCIENCIAVicerrectorado de Investigación y Posgrado - UNMSM B2110007i
6 · The paper itself

Abstract

RNA purification and cDNA synthesis represents the starting point for molecular analyses of snake venom proteins-enzymes. Usually, the sacrifice of snakes is necessary for venom gland extraction to identify protein-coding transcripts; however, the venom can be used as a source of transcripts. Although there are methods for obtaining RNA from venom, no comparative analysis has been conducted in the

Indexed as

BothropsCrotalid VenomsAnimalsClinical RelevanceDNA, ComplementaryPeruProteinsRNACrotalid VenomsDNA, ComplementaryProteinsRNABothropscDNA synthesisRNA purificationtranscriptsvenomvenom gland

Identifiers

PMID37446341
PMCPMC10342303
OpenAlexW4383551448

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.